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E6126

Enolase from baker′s yeast (S. cerevisiae)

lyophilized powder, ≥50 units/mg protein

Synonyme(s) :

2-Phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase

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A propos de cet article

Numéro CAS:
9014-08-8
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-754-3
MDL number:
MFCD00131019
Numéro CE :
4.2.1.11 (BRENDA, IUBMB)
Specific activity:
≥50 units/mg protein

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form

lyophilized powder

Quality Segment

200

specific activity

≥50 units/mg protein

mol wt

93 kDa

composition

Protein, 50-80% modified Warburg-Christian

solubility

15 mM Tris HCl, pH 7.4: soluble 1.0 mg/mL, clear

storage temp.

−20°C

General description

Enolase (2-phosphoglycerate hydratase) is a cytosolic enzyme. This cytosolic metalloenzyme is encoded by three homologous genes in mammals. Enolase the from baker′s yeast is a homodimer containing two bound Mg2+ ions. The peptide consists of 436 amino acids and contains a single cysteine residue and corresponds to a molecular weight of 93 kDa. Two of the active site components include His191 and Arg414. Enolase is seen in archaea, bacteria and eukaryotes.

Application

Enolase from baker′s yeast has been used in a study to investigate the contribution of the antibodies response induced by a low virulent Candida albicans strain in protection against systemic candidiasis. Enolase from baker′s yeast has also been used in a study to investigate the role of metal ions in catalysis by enolase.
Enolase from baker′s yeast (S. cerevisiae) has been used as a supplement in assay buffer for phosphoglycerate mutase (PGM) enzyme assays.
The enzyme from Sigma has been used as an antigen during ELISA. the study used human granulocyte proteins to identify and characterize autoantibodies against catalase and α-enolase in patients with primary sclerosing cholangitis.[1] It has been used to study temperature- and denaturant-induced yeast enolase denaturation using fourier transform infrared spectroscopy. It has also been used along with other proteins to study gradient chromatofocusing-mass spectrometry; a new technique for protein analysis.[2]

Biochem/physiol Actions

Enolase is a metalloenzyme that catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase is essential for both glycolysis and gluconeogenesis.
Enolase plays a key role in glycolysis and gluconeogenesis. Enolase, found in the nucleus of mammalian cells plays a key role in transcriptional modulation of genes, which participates in morphological transformation and cell proliferation.
The phosphorylated tyrosine residue present in yeast enolase forms a substrate for phosphorylation by tyrosine protein kinase.[3] Apart from Mg2+, the enzyme can be activated by Zn2+, Mn2+, and Cd2+.

Physical form

Lyophilized powder containing Tris buffer salts

Other Notes

One unit will convert 1.0 μmole of 2-phosphoglycerate to phospho(enol)pyruvate per min at pH 7.4 at 25 °C.

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Cet article
I1643P7634C3888
Enolase from baker’s yeast (S. cerevisiae) lyophilized powder, ≥50 units/mg protein

Sigma-Aldrich

E6126

Enolase from baker′s yeast (S. cerevisiae)

Pyrophosphatase, Inorganic from baker’s yeast (S. cerevisiae) powder, ≥500 units/mg protein (E1%/280)

Sigma-Aldrich

I1643

Pyrophosphatase, Inorganic from baker′s yeast (S. cerevisiae)

3-Phosphoglyceric Phosphokinase from baker’s yeast (S. cerevisiae) ammonium sulfate suspension, ≥500 units/mg protein

Sigma-Aldrich

P7634

3-Phosphoglyceric Phosphokinase from baker′s yeast (S. cerevisiae)

Carboxypeptidase Y from baker’s yeast (S. cerevisiae) lyophilized powder, ≥50 units/mg protein

Sigma-Aldrich

C3888

Carboxypeptidase Y from baker′s yeast (S. cerevisiae)

description

lyophilized powder, ≥50 units/mg protein

description

powder, ≥500 units/mg protein (E1%/280)

description

ammonium sulfate suspension, ≥500 units/mg protein

description

lyophilized powder, ≥50 units/mg protein

specific activity

≥50 units/mg protein

specific activity

≥500 units/mg protein (E1%/280)

specific activity

≥500 units/mg protein

specific activity

≥50 units/mg protein

form

lyophilized powder

form

powder

form

ammonium sulfate suspension

form

lyophilized powder

mol wt

93 kDa

mol wt

71 kDa

mol wt

-

mol wt

61 kDa

solubility

15 mM Tris HCl, pH 7.4: soluble 1.0 mg/mL, clear

solubility

-

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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