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E6909

ECM Gel from Engelbreth-Holm-Swarm murine sarcoma

growth-factor reduced, without phenol red, liquid, BioReagent, suitable for cell culture

Synonym(s):

GFR Basement Membrane Extract (BME)

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5 mL
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$463.00
10 mL
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$860.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00283131

$463.00

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Quality Level

200

product line

BioReagent

form

liquid

concentration

7-9 mg/mL

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P02468, P02469, P10493, P19137, P43407, Q05793, Q61001, Q61092, Q61789

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Hspg2(15530), Lama1(16772), Lama3(16774), Lama5(16776), Lamb1-1(16777), Lamc1(226519), Lamc2(16782), Nid1(18073), Sdc2(15529)

Application

ECM gel can be used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells.
ECM Gel from Engelbreth-Holm-Swarm murine sarcoma has been used to coat tissue culture plates for different glioma subtypes and C2C12 muscle cells. It has also been used as a scaffold to increase myotube attachment, maturation and survival.

Biochem/physiol Actions

The ECM gel will undergo thermally activated polymerization when brought to 20-40°C, forming a reconstituted basement membrane. PC12 cells will show neurite formation within 2 days when they are grown on a thin layer of this ECM gel.

Every mouse colony used for the production of this product is routinely screened for several pathogens. Tested and found negative for LDEV

Preparation Note

This product is prepared from Engelbreth-Holm-Swarm sarcoma in mice; dialyzed against chloroform. Gel should be thawed overnight at 2-8°C before use and dispensed to a multiwall plate using a plate and pipettes that are pre-cooled to 2-8°C. The gel may contain precipitates which do not influence its activity. The gel may also be diluted up to twofold with 2-8°C Dulbecco′s Modified Eagle′s Medium, and should be done before gel is added to the plate. The product will gel within 5 minutes at 20°C. For prolonged manipulations, work should be conducted below 10°C. Cells can be plated on top of a thin gel layer of 0.5 mm or cultured inside a 1 mm layer. If you culture cells inside, add the cells to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from this gel, use protease in PBS without calcium, magnesium, or EDTA, at a concentration of 0.6-2.4 units per mL.

Other Notes

ECM was prepared to a protein concentration of 8-12 mg/mL, containing laminin as a major component, collagen type IV, heparin sulfate proteoglycan, entactin, and other minor components. Addition of collagen type IV to the ECM gel increases polymerization.
The growth factor reduction modification allows for more control of medium, better interpretation of results and increased polymerization. The medium also lacks phenol red, which can interfere with some analyses.

Disclaimer

For long term storage, keep product at -20°C. The ECM gel may be stored at 2-8°C for up to 72 hours.

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This Item
E1270B1522D1145
ECM Gel from Engelbreth-Holm-Swarm murine sarcoma growth-factor reduced, without phenol red, liquid, BioReagent, suitable for cell culture

Sigma-Aldrich

E6909

ECM Gel from Engelbreth-Holm-Swarm murine sarcoma

ECM Gel from Engelbreth-Holm-Swarm murine sarcoma liquid, BioReagent, suitable for cell culture

Sigma-Aldrich

E1270

ECM Gel from Engelbreth-Holm-Swarm murine sarcoma

Basal Medium Eagle With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

SAFC

B1522

Basal Medium Eagle

Dulbecco’s Modified Eagle’s Medium - high glucose With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine, sodium pyruvate, and phenol red, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D1145

Dulbecco′s Modified Eagle′s Medium - high glucose

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

shipped in

dry ice

shipped in

ambient

shipped in

ambient

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

300

Quality Level

400

Quality Level

400

UniProt accession no.

P02468, P10493, P43407, Q61001, Q61789, P02469, Q05793, P19137, Q61092

UniProt accession no.

P02468, P02469, P10493, P19137, P43407, Q61001, Q61092, Q61789

UniProt accession no.

-

UniProt accession no.

-

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000537776
0000537778
0000537776
0000537778
0000474267

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Articles

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The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

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ECM Gel Matrix: Protocols Using EHS Basement Membrane Extracts

Cell culture protocols using ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.

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Global Trade Item Number

SKUGTIN
E6909-5ML04061833604755
E6909-10ML04061833604731

Questions

1–8 of 8 Questions  

  1. ECM cat:E6909 I would like to know if this product could be used in vivo to inject tumor cells into mouse liver.Its description says reduced growth factor but I would like to know if it contains anything, even in a low %,that can induce cell proliferation

    1 answer

    1. This product is not tested for in vivo use. The Quality testing does include neurite formation in cell culture. The end user would have to determine suitability for use in animal models. This ECM product undergoes an additional purification step which depleted growth factors. However, the material is not tested for the presence of growth factors post-purification. See the links below to review additional information regarding the presence and depletion of growth factors in Extracellular Matrix from mouse sarcoma:
      https://www.researchgate.net/publication/41454358_Matrigel_A_complex_protein_mixture_required_for_optimal_growth_of_cell_culture
      https://www.pnas.org/doi/epdf/10.1073/pnas.87.10.4002
      https://www.sciencedirect.com/science/article/pii/S1044579X05000313#bib16
      https://www.sciencedirect.com/science/article/abs/pii/001448279290397Q

      Helpful?

  2. What is the application of ECM for tube assay and the impact of reduced growth factors?

    1 answer

    1. I recommend the following publication for a comparison study on ECM vs ECM with reduced growth factor: J Vis Exp. 2016 Aug 14;(114):54074. doi: 10.3791/54074. Strategic Endothelial Cell Tube Formation Assay: Comparing Extracellular Matrix and Growth Factor Reduced Extracellular Matrix. PMID: 27585062.

      J Vis Exp. 2016 Aug 14;(114):54074. doi: 10.3791/54074. Strategic Endothelial Cell Tube Formation Assay: Comparing Extracellular Matrix and Growth Factor Reduced Extracellular Matrix. PMID: 27585062.

      Helpful?

  3. Is it possible to dissociate cells using Accutase when they are attached to the ECM gel E6909?

    1 answer

    1. Yes, it is possible to dissociate cells using Accutase when they are attached to the ECM gel E6909. It is advisable to start with a small-scale trial to assess its suitability.

      Helpful?

  4. Do Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, contain phenol red?

    1 answer

    1. Product E1270 ECM gel does contain phenol-red from the Dulbecco's modified Eagle's medium component of the product.  Product No. E6909, ECM gel, growth factor reduced, is phenol red free.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. Are Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, growth factor reduced like Matrigel (from BD)?

    1 answer

    1. Product No. E1270 is not growth factor reduced. Product No. E6909 is ECM gel, growth factor reduced.

      Helpful?

  7. Are ECM Gels, Products E1270 and E6909, the same as Matrigel (from BD)?

    1 answer

    1. Product No. E1270, ECM gel is the equivalent of Matrigel from BD only produced by Sigma-Aldrich.

      Helpful?

  8. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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