E8022
pRc/RSV Expression vector
set of expression vectors powered by either the CMV2 or the RSV promoter
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
20 μg | Comprobar disponibilidad del carrito | MXP 15,187.00 |
Acerca de este artículo
MXP 15,187.00
Comprobar disponibilidad del carrito
Servicio técnico
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Molecular Biology
form
buffered aqueous solution
storage temp.
−20°C
General description
pRc and pRSV Supercoiled Expression Vectors (5.2 kb) are for protein expression in mammalian cells. Each vector contains:
The primary difference between these expression vectors is that pRc contains the enhancer-promoter sequence from human cytomegalovirus (CMV2) and pRSV from Rous sarcoma virus (RSV). Both are strong promoters for high-level transcription.
The pRc MCS contains unique ApaI, HindIII, NotI, and XbaI restriction sites, plus redundant BstXI sites.
The pRSV MCS contains unique HindIII, NotI, SpeI, and XbaI restriction sites, plus redundant BstXI sites.
- The polyadenylation signal and transcription termination sequence from BGH to enhance mRNA stability
- The SV40 replication origin for episomal replication
- The f1 origin for rescue of the sense strand
- The neomycin resistance gene, and
- An ampicillin resistance gene and colE1 origin for selection and maintenance in E. coli.
The primary difference between these expression vectors is that pRc contains the enhancer-promoter sequence from human cytomegalovirus (CMV2) and pRSV from Rous sarcoma virus (RSV). Both are strong promoters for high-level transcription.
The pRc MCS contains unique ApaI, HindIII, NotI, and XbaI restriction sites, plus redundant BstXI sites.
The pRSV MCS contains unique HindIII, NotI, SpeI, and XbaI restriction sites, plus redundant BstXI sites.
Biochem/physiol Actions
The MCS contains unique ApaI (CMV2 only), HindIII, NotI, SpeI (RSV only), and XbaI restriction sites, plus redundant BstXI sites.
Features and Benefits
Supercoiled vectors for protein expression in mammalian cells. Both of these vectors contain:
- the polyadenylation signal and transcription termination sequence from BGH to enhance mRNA stability
- the SV40 replication origin for episomal replication
- the f1 origin for rescue of the sense strand
- the neomycin resistance gene, and
- an ampicillin resistance gene and colE1 origin for selection and maintenance in E. coli.
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Este artículo | ||
|---|---|---|
| form buffered aqueous solution | form - | form - |
| grade Molecular Biology | grade - | grade Molecular Biology |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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