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Trypsin Protease

Name: Trypsin Protease
Brand: SIGMA
Price: 98 USD
Availability: InStock

Hydrolyzes peptide bonds at the carboxyl side of arginine and lysine residues, suitable for mass spectrometry, recombinant, expressed in Pichia pastoris

Synonym(s):

Mass Spectrometry Trypsin, Proteomics grade Trypsin, rTrypsin

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Size/SKU	Availability	Price
100 μg	Estimated to ship TODAYfromSAINT LOUIS	$98.00
4 x 100 μg	Estimated to ship TODAYfromSAINT LOUIS	$345.00

About This Item

UNSPSC Code:

12352204

NACRES:

NA.26

Specific activity:

≥10,000 units/mg protein

Recombinant:

expressed in Pichia pastoris

$98.00

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Properties

Product Name

Recombinant Trypsin, Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris

recombinant

expressed in Pichia pastoris

Quality Segment

200

grade

Proteomics Grade

form

lyophilized powder

specific activity

≥10,000 units/mg protein

technique(s)

protein mass spectrometry: suitable

suitability

suitable for , suitable for mass spectrometry

UniProt accession no.

P00761

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Trypsin is a major proteolytic enzyme, synthesized as a preproenzyme by pancreas and is stored as proenzyme trypsinogen in secretory granules. Trypsin belongs to the family of serine proteases that are characterized by the catalytic triad His57, Asp102 and Ser195. Trypsin is routinely used in proteomics research for peptide mapping and protein sequence work due to its highly specific cleavage resulting in a limited number of tryptic peptides. Trypsin is a pancreatic serine endoprotease which hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues. The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage site and cleavage may not occur if a proline residue is on the carboxyl side. The enzyme also exhibits esterase and amidase activities. Trypsin has an average molecular mass of 23.29 kDa and a pH optimum near 8.0. This product is prepared from recombinant trypsin, porcine sequence. It is naturally devoid of chymotryptic activity. This high-quality trypsin is suitable for proteomics use.

Biochem/physiol Actions

Trypsin plays an important role in the digestion of consumed protein and contributes to the activation of other proteolytic enzymes like chemotrypsin and elastase.

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This Item	EMS0007	EMS0004	EMS0005
Sigma-Aldrich EMS0006 Recombinant Trypsin	Sigma-Aldrich EMS0007 Recombinant Trypsin Dimethlyated	Sigma-Aldrich EMS0004 SOLu-Trypsin	Sigma-Aldrich EMS0005 SOLu-Trypsin Dimethylated
description Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris	description Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris	description recombinant, expressed in Pichia pastoris, Proteomics Grade, liquid	description Dimethlyated, Proteomics Grade, recombinant, expressed in Pichia pastoris, ready-to-use solution
specific activity ≥10,000 units/mg protein	specific activity ≥10,000 units/mg protein	specific activity -	specific activity ≥10,000 units/mg protein (Enzymatic activity)
technique(s) protein mass spectrometry: suitable	technique(s) protein mass spectrometry: suitable	technique(s) protein mass spectrometry: suitable	technique(s) protein mass spectrometry: suitable
grade Proteomics Grade	grade Proteomics Grade	grade Proteomics Grade	grade Proteomics Grade
form lyophilized powder	form lyophilized powder	form ready-to-use solution	form ready-to-use solution
suitability suitable for mass spectrometry	suitability suitable for mass spectrometry	suitability suitable for mass spectrometry	suitability suitable for mass spectrometry
recombinant expressed in Pichia pastoris	recombinant expressed in Pichia pastoris	recombinant expressed in Pichia pastoris	recombinant expressed in Pichia pastoris

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Pictograms

GHS08,GHS05,GHS07,GHS09

Signal Word

Danger

Hazard Codes

H290,H314,H317,H334,H335,H411

Precautionary Statements

P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

supp_hazards

EUH071

Storage Class

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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