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F0137

Fructose-6-phosphate Kinase from Bacillus stearothermophilus

Type VII, lyophilized powder, ≥50 units/mg protein

Synonyme(s) :

6-Phosphofructokinase, ATP:D-fructose 6-phosphate 1-phosphotransferase

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100 units
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409,00 $
250 units
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880,00 $

A propos de cet article

Numéro CAS:
9001-80-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-633-5
MDL number:
MFCD00131107
Numéro CE :
2.7.1.11 (BRENDA, IUBMB)
Specific activity:
≥50 units/mg protein

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type

Type VII

Quality Segment

200

form

lyophilized powder

specific activity

≥50 units/mg protein

mol wt

34 kDa

shipped in

wet ice

storage temp.

−20°C

General description

Research Area: Cell Signaling
Bacillus stearothermophilus phosphofructokinase (BsPFK) is a homotetramer that is allosterically inhibited by phosphoenolpyruvate (PEP), which binds along one dimer-dimer interface.[1]
Fructose-6-phosphate kinase from Bacillus stearothermophilus, a 34 kDa enzyme, belongs to phosphofructokinase (PFK) - like superfamily. The glutamate 161 and arginine 162 residues at the active site are crucial for substrate binding.[2]

Application

Fructose-6-phosphate Kinase from Bacillus stearothermophilus was shown to interact with neuronal nitric oxide synthase (nNOS) causing a defect in glycolytic metabolism and increased fatigability in dystrophic muscle.[3]
Fructose-6-phosphate Kinase from Bacillus stearothermophilus has been used in the assay mixture for mass spectrometry assay for phosphoglucoisomerase (PGI) (G6P to F6P reaction).[4]
Fructose-6-phosphate kinase from Bacillus stearothermophilus has been used:
  • in steady state analysis of phosphofructokinase activity in the presence of ATP deuterated at the C8 position(C8-D ATP)
  • for standard curve generation for quantifying muscle phosphofructokinase (PFK) activity

Biochem/physiol Actions

Fructose-1,6-bisphosphatase (FBP) is an important enzyme in glucose metabolism. It catalyzes the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate.[5] Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle.[6]
Phosphofructokinase (PFK) is an essential bifunctional enzyme that serves as a critical regulator in the intermediate stages of glycolysis. PFK is strongly linked to caveolae and is brought to caveolae by caveolin-1 in vascular smooth muscle cells (VSMCs).

Physical form

Lyophilized powder containing buffer salt (e.g. phosphate buffer, or Tris buffer with NaCl)

Other Notes

One unit will convert 1.0 μmole of fructose 6-phosphate and ATP to fructose 1,6-diphosphate and ADP per minute at pH 9.0 at 30 °C.

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Cet article
P2783P5538A7437
Fructose-6-phosphate Kinase from Bacillus stearothermophilus Type VII, lyophilized powder, ≥50 units/mg protein

Sigma-Aldrich

F0137

Fructose-6-phosphate Kinase from Bacillus stearothermophilus

Phosphotransacetylase from Bacillus stearothermophilus lyophilized powder, ≥3,000 units/mg protein (biuret)

Sigma-Aldrich

P2783

Phosphotransacetylase from Bacillus stearothermophilus

Phosphoglucose Isomerase from Bacillus stearothermophilus lyophilized powder, 300-1,000 units/mg protein

Sigma-Aldrich

P5538

Phosphoglucose Isomerase from Bacillus stearothermophilus

Acetate Kinase from Escherichia coli lyophilized powder, ≥150 units/mg protein (biuret)

Sigma-Aldrich

A7437

Acetate Kinase from Escherichia coli

description

Type VII, lyophilized powder, ≥50 units/mg protein

description

lyophilized powder, ≥3,000 units/mg protein (biuret)

description

lyophilized powder, 300-1,000 units/mg protein

description

lyophilized powder, ≥150 units/mg protein (biuret)

specific activity

≥50 units/mg protein

specific activity

≥3,000 units/mg protein (biuret)

specific activity

300-1,000 units/mg protein

specific activity

≥150 units/mg protein (biuret)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

34 kDa

mol wt

-

mol wt

189 kDa

mol wt

-

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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