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Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit

Name: Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit
Brand: SIGMA
Price: 801 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Enhanced Validation

Synonym(s):

Anti-FGFR-2

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Size/SKU	Availability	Price
0.5 mL	Available to ship TODAYfromMILWAUKEE	$801.00

About This Item

UNSPSC Code:

51111800

NACRES:

NA.41

MDL number:

MFCD02096344

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC (p), WB

Citations:

$801.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 115-125 kDa (doublet)

species reactivity

human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using trypsin-digested, human tissue sections, western blot: 0.25-0.5 μg/mL using extracts/lysates of FGFR-2 transfected cells

UniProt accession no.

P21802

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FGFR2(2263)

Description

General description

Fibroblast growth factors receptors (FGFRs) are members of the tyrosine kinase family of growth factor receptors. They are glycosylated 110-150 k Da proteins that are constructed of an extracellular ligand binding region with either two or typically three immunoglobulin (Ig)-like domains and an eight amino acid ′acidic box′, a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. At the mRNA level, FGFR-2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It is widely expressed in many adult human and animal tissues. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells.

Immunogen

synthetic peptide corresponding to amino acids 809-821 of the cytoplasmic region of human FGFR-2 (bek).

Application

Anti-Fibroblast Growth Factor Receptor-2 has been used in immunoblotting, immunoprecipitation, immunohistochemistry,western blotting.

Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit was used for immunohistochemistry of free-floating rat brain tissue sections.

Biochem/physiol Actions

Fibroblast growth factors (FGFs) and their receptors, FGFRs, are important regulators of cell proliferation, migration, tissue repair and differentiation during vertebrate development. FGFR-2 isoforms are expressed exclusively on cells of epithelial origin and are involved in tumor progression in thyroid cancers. Abnormalities in FGFR-2 expressions have been observed in skin, bone and cancer pathologies.

Fibroblast growth factors receptors (FGFRs) mediated signal transduction requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Mutations in the extracellular domain of FGFR-2 causes Crouzon, as well as other craniosynostosis syndromes (e.g. Pfeiffer′s, Apert′s, Jackson-Weiss′), disorders of human skeletal development.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	F6796	SAB4500889	F6162
Sigma-Aldrich F0300 Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit	Sigma-Aldrich F6796 Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit	Sigma-Aldrich SAB4500889 Anti-FGFR2 antibody produced in rabbit	Sigma-Aldrich F6162 Anti-Fibroblast Growth Factor-Basic antibody, Mouse monoclonal
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody	description clone FB-8, purified from hybridoma cell culture
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified immunoglobulin
biological source rabbit	biological source rabbit	biological source rabbit	biological source mouse
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 200
technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using trypsin-digested, human tissue sections, western blot: 0.25-0.5 μg/mL using extracts/lysates of FGFR-2 transfected cells	technique(s) western blot: 1:2,000 using extract of FGFR-2 transiently transfected cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using trypsin-digested, human and animal tissue sections	technique(s) immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000, ELISA: 1:10000	technique(s) indirect ELISA: suitable, western blot: suitable, radioimmunoassay: suitable, neutralization: 5 μg/mL using 1 unit recombinant human FGF2 in mouse 3T3 cells, dot blot: suitable
UniProt accession no. P21802	UniProt accession no. P21802	UniProt accession no. P21802	UniProt accession no. P09038

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Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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