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F0397

Anti-Cytokeratin pan-FITC antibody, Mouse monoclonal

clone PCK-26, purified from hybridoma cell culture

Sinônimo(s):

Anti-CK16-Fluorescein isothiocyanate, Anti-FNEPPK, Anti-K16, Anti-K1CP, Anti-KRT16A, Anti-NEPPK, Anti-PC1

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Para você/SKUDisponibilidadePreço
0.2 mL
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R$ 3.631,00
R$ 3.086,35

Sobre este item

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
FITC conjugate
Clone:
PCK-26, monoclonal
Application:
IF (d), IHC (f)
Citations:
16

R$ 3.086,35

Preço de tabelaR$ 3.631,00Poupe 15%
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biological source

mouse

Quality Segment

200

conjugate

FITC conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

PCK-26, monoclonal

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:25 using formalin-fixed, paraffin-embedded sections of human or animal tissues, immunohistochemistry (frozen sections): suitable

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Cytokeratins are proteins of keratin-containing intermediate filaments that provide mechanical support and other additional functions in epithelial cells. It is found in the intracytoplasmic cytoskeleton of epithelial tissue. Epithelial tissue expresses cytokeratin subunits in a specific and stable pattern. Cytokeratins along with vimentin are involved in cell proliferation, migration and differentiation of preodontoblasts and preameloblasts. The intermediate-sized filaments are abundant in human endothelial cells and are mostly of vimentin type.
FITC Monoclonal Anti-Pan Cytokeratin is a purified mouse monoclonal antibody conjugated with fluorescein isothiocyanate (FITC) isomer I. Monoclonal anti-pan cytokeratin (mouse IgG1 isotype) is derived from the PCK-26 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice. Cytokeratins, a group comprised of at least 29 different proteins are characteristic of epithelial and trichocytic cells. Cytokeratin 5, 6 and 8 are members of the type II, neutral-to-basic subfamily. Cytokeratin peptide 5 is the primary type II keratin in stratified epithelia while cytokeratin type 8 is a major type II keratin in simple epithelia. Cytokeratin 6 is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover.

Immunogen

cytokeratin from human epidermis.

Application

Monoclonal Anti-Cytokeratin, pan-FITC antibody produced in mouse has been used in:
  • immunohistochemistry
  • staining chicken intestinal cells
  • immunofluorescence staining

Monoclonal Anti-Cytokeratin, pan-FITC antibody produced in mouse is suitable for direct immunofluorescence at a dilution of 1:25 using formalin-fixed, paraffin-embedded sections of human or animal tissues and immunohistochemistry (frozen sections).

Biochem/physiol Actions

The antibody recognizes an epitope located on the Type II cytokeratins 1, 5, 6, and 8. PCK-26 is a broad spectrum antibody which reacts specifically with a variety of normal, reactive, and neoplastic epithelial tissues. The antibody reacts with simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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F3418F4772SAB4200855
Anti-Cytokeratin pan-FITC antibody, Mouse monoclonal clone PCK-26, purified from hybridoma cell culture

Sigma-Aldrich

F0397

Anti-Cytokeratin pan-FITC antibody, Mouse monoclonal

Monoclonal Anti-Cytokeratin, pan−FITC antibody produced in mouse clone C-11, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

F3418

Monoclonal Anti-Cytokeratin, pan−FITC antibody produced in mouse

Monoclonal Anti-Cytokeratin Peptide 18−FITC antibody produced in mouse clone CY-90, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

F4772

Monoclonal Anti-Cytokeratin Peptide 18−FITC antibody produced in mouse

Anti-Cytokeratin Peptide 18 antibody, Mouse monoclonal clone CY-90, purified from hybridoma cell culture

Sigma-Aldrich

SAB4200855

Anti-Cytokeratin Peptide 18 antibody, Mouse monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

-

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

unconjugated

clone

PCK-26, monoclonal

clone

C-11, monoclonal

clone

CY-90, monoclonal

clone

CY-90, monoclonal

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

technique(s)

direct immunofluorescence: 1:25 using formalin-fixed, paraffin-embedded sections of human or animal tissues, immunohistochemistry (frozen sections): suitable

technique(s)

direct immunofluorescence: 1:50 using PtK2 cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human placenta, western blot: suitable

technique(s)

direct immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded sections of human or animal tissues, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

technique(s)

immunoblotting: suitable, immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human placenta sections

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

liquid

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flash_point_c

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Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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