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F1763

Anti-Rat IgG (whole molecule)–FITC antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Rabbit Anti-Rat IgG (whole molecule)–Fluorescein isothiocyanate

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1 mL

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$248.00
$210.80

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162811
Conjugate:
FITC conjugate
Clone:
polyclonal
Application:
IF, IHC (p)
Citations:
35

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biological source

rabbit

Quality Level

200

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320, indirect immunofluorescence: 1:320

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-Rat IgG (whole molecule) is produced in rabbit using purified rat IgG as the immunogen.
Rat IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rat serum. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Anti-Rat IgG (whole molecule)-FITC antibody binds to all rat immunoglobulins.

Immunogen

Purified rat IgG

Application

Anti-Rat IgG (whole molecule)-FITC antibody has been used:
  • in immunolabelling for confocal laser scanning microscopy (CLSM)
  • in immunostaining

Anti-Rat IgG (whole molecule)-FITC antibody is suitable for use in flow cytometry (1:100) and immunofluorescence.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Immunofluorescence (1 paper)
Rabbit polyclonal anti-Rat IgG (whole molecule)-FITC antibody may be used to detect and quantitate the level of IgG in rat serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use pig IgG as the primary antibody.

Biochem/physiol Actions

IgGs are glycoprotein antibodies that modulate several immune responses. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins.
Rabbit polyclonal anti-Rat IgG (whole molecule)−FITC antibody reacts with rat IgG in vitro, in rat serum and biological fluids.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with human samples.

Other Notes

Antibody adsorbed with human IgG.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Rat IgG (whole molecule)–FITC antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

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Anti-Bovine IgG (whole molecule)–FITC antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

F7887

Anti-Bovine IgG (whole molecule)–FITC antibody produced in rabbit

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

rabbit

biological source

goat

biological source

goat

biological source

rabbit

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320, indirect immunofluorescence: 1:320

technique(s)

direct immunofluorescence: 1:32

technique(s)

indirect immunofluorescence: 1:80

technique(s)

direct immunofluorescence: 1:300, immunohistochemistry: 1:300

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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Certificates of Analysis (COA)

Lot/Batch Number
0000456730
0000456730
0000456716
0000456716
0000416923

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Global Trade Item Number

SKUGTIN
F1763-2ML04061837516498
F1763-1ML04061837516481
F1763-.5ML04061833612484

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