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F2653
Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat
affinity isolated antibody, buffered aqueous solution
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.5 mL | Available to ship TODAYfromMILWAUKEE | $374.00 |
About This Item
Conjugate:
FITC conjugate
Clone:
polyclonal
Application:
FACS, IHC (p)
Citations:
16
biological source
goat
Quality Level
conjugate
FITC conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
flow cytometry: 1:100, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160
storage temp.
−20°C
target post-translational modification
unmodified
General description
IgG, a monomer, is the principal antibody circulating in blood which assist in phagocytic destruction of foreign microorganism and is the only class of Ig that can cross the placenta in humans. Cell-sorting done by differential binding of anti-mouse IgG (Fab Specific) - FITC antibody separates tumour cells from macrophages. This antibody can be used to incubate somatic cell nuclear transfer (SCNT) rat embryos for immunocytochemical observation. It also can be used as a secondary antibody to study the dynamics of activated B lymphocytes in small intestine, Peyer′s patch germinal center as well as non-germinal center, spleen and the mesenteric lymph node. Anti-mouse IgG antibody reacts specifically with all mouse immunoglobulins.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Immunogen
Purified Fab fragments of mouse IgG.
Application
Anti-Mouse IgG (Fab Specific) FITC Conjugate antibody is used in traditional method of immunocytochemical assessment of ZAP-70 expression. It can also be used in immunocytochemical observation of the SCNT embryos.
Anti-Mouse IgG (Fab specific) F(ab′)2 fragment-FITC antibody produced in goat has been used in:
- indirect immunofluorescence staining
- immunofluorescence staining
- flow cytometry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Flow cytometry/Cell sorting (1 paper)
Biochem/physiol Actions
Binds all mouse Igs.
Useful when trying to avoid background staining due to the presence of Fc receptors.
Useful when trying to avoid background staining due to the presence of Fc receptors.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note
Adsorbed to reduce background staining with bovine, horse or human samples.
Other Notes
Antibody adsorbed with bovine, equine and human serum proteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| biological source goat | biological source goat | biological source goat | biological source goat |
| conjugate FITC conjugate | conjugate FITC conjugate | conjugate FITC conjugate | conjugate FITC conjugate |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| technique(s) flow cytometry: 1:100, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, indirect immunofluorescence: 1:128 | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320 | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, indirect immunofluorescence: 1:32 |
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Datasheet
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| F2653-.5ML | 04061837516566 |
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What applications have product F2653, Anti-Mouse IgG (Fab specific) F(ab´)2 fragment-FITC antibody produced in goat, been used in?
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Product F2653, Anti-Mouse IgG (Fab specific) F(ab´)2 fragment-FITC antibody produced in goat, has been used in Flow Cytometry in the following literature references.Bergman, I. et al., Clinical Cancer Research., 7, 2050 (July 2001).Farsinejad, A. et al. Iranian Journal of Blood and Medicine, 2, Number 3, (Spring 2010).This antibody has been used in Immunofluorescencs in the following literature reference.Sushan, A., Hum. Reprod., 19 (9) : 1957-1967 (2004).
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