F2883
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–FITC antibody produced in sheep
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-Mouse IgG FITC
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.5 mL | Available to ship TODAYfromMILWAUKEE | $192.00 | |
| 1 mL | Available to ship TODAYfromMILWAUKEE | $332.00 $282.20 | |
| 2 mL | Available to ship TODAYfromMILWAUKEE | $538.00 |
About This Item
Conjugate:
FITC conjugate
Clone:
polyclonal
Application:
IF (d)
Citations:
31
biological source
sheep
Quality Level
conjugate
FITC conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct immunofluorescence: 1:128
storage temp.
2-8°C
target post-translational modification
unmodified
General description
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-FITC antibody is specific for ) F(ab′)2 fragment of mouse IgG1, G2a, G2b, and G3 subclasses. The antibody preparation is solid phase adsorbed with human serum proteins prior to conjugation to minimize cross reactivity. The antibody preparation is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment is specific for mouse IgG subclasses G1, G2a, G2b, and G3 as demonstrated by Ouchterlony double diffusion using mouse myeloma proteins. The antibody product can be used to avoid background staining due to the presence of Fc receptors.
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment is specific for mouse IgG subclasses G1, G2a, G2b, and G3 as demonstrated by Ouchterlony double diffusion using mouse myeloma proteins. The antibody product can be used to avoid background staining due to the presence of Fc receptors.
Immunogen
Mouse IgG
Pepsin digest of antiserum
Application
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-FITC antibody may be used in direct immunofluorescence at a minimum antibody dilution of 1:128. The antibody was used at a working dilution of 1:25 to stain microtubules in epidermal cells of Vigna unguiculata or cowpea It was also used for flow cytometry analysis using COS cells and human peripheral blood mononuclear cells.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Chromatin immunoprecipitation (1 paper)
Biochem/physiol Actions
Binds all mouse Igs.
Useful when trying to avoid background staining due to the presence of Fc receptors.
Useful when trying to avoid background staining due to the presence of Fc receptors.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note
Adsorbed to reduce background staining with human samples.
Other Notes
Antibody adsorbed with human serum proteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| biological source sheep | biological source sheep | biological source sheep | biological source sheep |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| conjugate FITC conjugate | conjugate FITC conjugate | conjugate FITC conjugate | conjugate CY3 conjugate |
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| technique(s) direct immunofluorescence: 1:128 | technique(s) direct immunofluorescence: 1:32 | technique(s) direct immunofluorescence: 1:128 | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 |
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Storage Class
12 - Non Combustible Liquids
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Related Content
Datasheet
D Skalamera et al.
The Plant journal : for cell and molecular biology, 16(2), 191-200 (1998-10-01)
During the infection of cowpea (Vigna unguiculata) by the cowpea rust fungus (Uromyces vignae, race 1 ) the plant nucleus moves towards and away from the invading hypha and eventually moves close to the fungus in the susceptible cultivar while
J R Huth et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(10), 5231-5236 (2000-05-11)
The leukocyte integrin, lymphocyte function-associated antigen 1 (LFA-1) (CD11a/CD18), mediates cell adhesion and signaling in inflammatory and immune responses. To support these functions, LFA-1 must convert from a resting to an activated state that avidly binds its ligands such as
Anne-Sophie Gabet et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 22(2), 622-632 (2007-09-28)
The skin human papillomavirus (HPV) types belonging to the genus beta of the HPV phylogenetic tree appear to be associated with nonmelanoma skin cancer. We previously showed that the beta HPV type 38 E6 and E7 oncoproteins are able to
Global Trade Item Number
| SKU | GTIN |
|---|---|
| F2883-1ML | 04061837516597 |
| F2883-.5ML | 04061837516580 |
| F2883-2ML | 04061833613382 |