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F4137

Anti-Chicken IgY (IgG) (whole molecule)−FITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Rabbit Anti-Chicken IgY (IgG) (whole molecule)−Fluorescein isothiocyanate

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1 mL

Available to ship TODAYfromMILWAUKEE

$210.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162224
Conjugate:
FITC conjugate
Clone:
polyclonal
Application:
IF, IHC (p)
Citations:
12

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biological source

rabbit

Quality Level

200

conjugate

FITC conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, indirect immunofluorescence: 1:200

storage temp.

−20°C

target post-translational modification

unmodified

General description

Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa.
Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus chicken antibodies are useful analytical tools for various research applications .

Immunogen

Chicken IgY (IgG)
Purified chicken IgY (IgG)

Application

Anti-Chicken IgY (IgG) (whole molecule) FITC antibody has been used:
  • in indirect immunofluorescence
  • in western blotting
  • in immunocytochemistry

Biochem/physiol Actions

Birds, reptiles and amphibians express IgY antibodies that are functionally equivalent to IgG. Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for IgY of chicken. Rabbit Anti- Chicken IgY (IgG) is conjugated to fluorescein isothiocyanate (FITC). Free FITC is removed by gel filtration.
IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Chicken IgY (IgG) (whole molecule)−FITC antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

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Sigma-Aldrich

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Sigma-Aldrich

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Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

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Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

FITC conjugate

conjugate

peroxidase conjugate

conjugate

FITC conjugate

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, indirect immunofluorescence: 1:200

technique(s)

direct ELISA: 1:30,000, dot blot: 1:160,000 (indirect chemiluminescence), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

technique(s)

direct immunofluorescence: 1:320 using human peripheral blood lymphocytes, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320 using human tonsil

technique(s)

direct ELISA: 1:30,000, dot blot: 1:160,000 using indirect chemiluminescence system using 5 ng human IgG/dot and chicken anti-human IgG as the primary antibody and Luminol plus enhancer as the substrate., immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using using human pancreas sections and chicken anti-human insulin as the primary antibody.

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000340623
0000340623
0000284776
0000268216
0000265903

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Related Content

Beatriz Maria de Almeida Braz et al.
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria, 29(4), e014120-e014120 (2020-10-22)
Toxoplasmosis is a parasitic disease, which is prevalent across the world and has a strong environmental component in its transmission chain. The present study determined the seroprevalence and factors related to Toxoplasma gondii infection among free-range chickens from an Atlantic
Aymn T Abbas et al.
Vaccines, 8(4) (2020-11-04)
The Middle East respiratory syndrome coronavirus (MERS-CoV) was identified in 2012 and causes severe and often fatal acute respiratory illness in humans. No approved prophylactic and therapeutic interventions are currently available. In this study, we have developed egg yolk antibodies
Lineage conversion induced by pluripotency factors involves transient passage through an iPSC stage
Bar-Nur O, et al.
Nature Biotechnology, 33(7), 761-761 (2015)
View All Related Papers

Global Trade Item Number

SKUGTIN
F4137-1ML04061833617540

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