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F4890

Monoclonal Anti-Rabbit Immunoglobulins–FITC antibody produced in mouse

clone RG-16, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)

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0.2 mL
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$339.00

About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
FITC conjugate
Clone:
RG-16, monoclonal
Application:
IF, IHC (p)
Citations:
17

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biological source

mouse

Quality Level

200

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

RG-16, monoclonal

form

buffered aqueous solution

should not react with

human, pig, chicken, bovine, horse, guinea pig, turkey, rat, canine, goat, feline, sheep

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, indirect immunofluorescence: 1:50 using using chicken fibroblast cells and primary antibody produced in rabbit

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

Application

Monoclonal Anti-Rabbit Immunoglobulins-FITC antibody produced in mouse has been used in:
  • immunocytochemistry
  • immunohistochemistry
  • immunofluorescence

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

Biochem/physiol Actions

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

Physical form

Solution in 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
F4891B3275B5283
Monoclonal Anti-Rabbit Immunoglobulins–FITC antibody produced in mouse clone RG-16, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

F4890

Monoclonal Anti-Rabbit Immunoglobulins–FITC antibody produced in mouse

Anti-Goat/Sheep IgG−FITC antibody, Mouse monoclonal clone GT-34, purified from hybridoma cell culture

Sigma-Aldrich

F4891

Anti-Goat/Sheep IgG−FITC antibody, Mouse monoclonal

Monoclonal Anti-Rabbit Immunoglobulins–Biotin antibody produced in mouse clone RG-16, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

B3275

Monoclonal Anti-Rabbit Immunoglobulins–Biotin antibody produced in mouse

Anti-Rabbit IgG (γ-chain specific)−Biotin antibody, Mouse monoclonal clone RG-96, purified from hybridoma cell culture

Sigma-Aldrich

B5283

Anti-Rabbit IgG (γ-chain specific)−Biotin antibody, Mouse monoclonal

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

biotin conjugate

conjugate

biotin conjugate

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, indirect immunofluorescence: 1:50 using using chicken fibroblast cells and primary antibody produced in rabbit

technique(s)

dot immunobinding: 1:32, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, particle immunofluorescence: 1:32

technique(s)

direct ELISA: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500, western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)

technique(s)

direct ELISA: 1:150,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000, western blot (chemiluminescent): 1:200,000-1:400,000

clone

RG-16, monoclonal

clone

GT-34, monoclonal

clone

RG-16, monoclonal

clone

RG-96, monoclonal

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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Storage Class

10 - Combustible liquids

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flash_point_f

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flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000417623
0000417623
0000324929
0000324929
0000236948

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Related Content

Data Sheet - F4890

Instructions

Carole Ntayi et al.
The Journal of investigative dermatology, 122(2), 256-265 (2004-03-11)
Type I collagen mediates melanoma cells invasion through upregulation of matrix metalloproteinases-1 and -2 (MMP-1 and -2) expression and activation. We investigated here the contribution of elastin-derived peptides (ED), degradation products of elastin, the main component of elastic fibers in
Timofey Lebedev et al.
Oncogene, 40(44), 6258-6272 (2021-09-25)
Neuroblastoma (NB) has a low frequency of recurrent mutations compared to other cancers, which hinders the development of targeted therapies and novel risk stratification strategies. Multikinase inhibitors have shown potential in treating high-risk NB, but their efficacy is likely impaired
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Poulsen FR, et al.
Experimental Brain Research. Experimentelle Hirnforschung. Experimentation Cerebrale, 135(3), 319-330 (2000)
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Global Trade Item Number

SKUGTIN
F4890-.2ML04061838354471

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