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Anti-Mouse IgG (Fab specific)−FITC antibody produced in goat

Name: Anti-Mouse IgG (Fab specific)−FITC antibody produced in goat
Brand: SIGMA
Price: 189 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Mouse IgG (Fab specific)−Fluorescein isothiocyanate

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Pack Size	SKU	Availability	Price
0.5 mL		Available to ship TODAYfromMILWAUKEE	$189.00
1 mL		Available to ship TODAYfromMILWAUKEE	$301.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162622

Conjugate:

FITC conjugate

Clone:

polyclonal

Application:

IF, IHC (p)

Citations:

$189.00

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Properties

biological source

goat

Quality Level

200

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150, indirect immunofluorescence: 1:128

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Mouse IgG (Fab specific)-FITC antibody is specific for Fab fragment of all mouse IgG subclasses. It shows no reactivity with mouse IgG, Fc fragment, or human IgG. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC. The antibody is adsorbed with human IgG to ensure minimal cross reactivity.

Immunoglobulins (Igs) belong to the immunoglobulin super-family. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4.. The IgG heavy chain region is mapped to human chromosome 14. The immunoglobulin structure comprises of two heavy (H) and two light (L) chains, held together by disulfide linkages. The heavy chain has one variable N-terminal region and three to four constant (C_H1-C_H4) C-terminal regions. The L chain comprises of one variable N-terminal region and a constant C-terminal region.

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

Immunogen

Purified mouse IgG, Fab fragment

Application

Anti-Mouse IgG (Fab specific) FITC antibody produced in goat has been used in:

immunolocalization of pancreases or isolated islets
immunocytochemistry analysis human tumor cell lines
immunohistochemistry of articular cartilage secondary antibody

Anti-Mouse IgG (Fab specific)-FITC antibody may be used for immunofluorescence of human peripheral blood lymphocytes at a minimum dilution of 1:128. It may be used for imunohistochemistry of formalin-fixed, paraffin-embedded human tonsil slides at a working antibody dilution of 1:150. In an agar diffusion assay the conjugate produces a precipitation arc at a dilution of 1:32 versus a 1:80 dilution of normal mouse serum. The antibody was used at a dilution range of 1:50 to 1:300 for immunofluorescence in various studies.

Biochem/physiol Actions

IgG1 class is the most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions. IgG4 is associated with asymptomatic infection. Digestion of IgG by papain results in generation of fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallizable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies.

Specificity for the Fab fragment of mouse IgG is determined by immunoelectrophoresis (IEP). The conjugate shows no reactivity with mouse IgG, Fc fragment, or human IgG.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with human samples.

For continuous use, store at 2-8 °C for a maximum of one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	F4018	F5897	F5387
Sigma-Aldrich F5262 Anti-Mouse IgG (Fab specific)−FITC antibody produced in goat	Sigma-Aldrich F4018 Anti-Mouse IgG (Fab specific)−FITC antibody produced in goat	Sigma-Aldrich F5897 Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat	Sigma-Aldrich F5387 Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat
biological source goat	biological source goat	biological source goat	biological source goat
conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150, indirect immunofluorescence: 1:128	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, indirect immunofluorescence: 1:64	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, indirect immunofluorescence: 1:64	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, indirect immunofluorescence: 1:32
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Global Trade Item Number

SKU	GTIN
F5262-1ML	04061833618165
F5262-.5ML	04061833618158

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