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F6257

Anti-Mouse IgG (whole molecule)–FITC antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Sheep Anti-Mouse IgG (whole molecule)–Fluorescein isothiocyanate

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0.5 mL

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$87.90
$74.71
1 mL

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$345.00
$293.25
2 mL

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
Conjugate:
FITC conjugate
Clone:
polyclonal
Application:
IF (d)
Citations:
26

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biological source

sheep

Quality Level

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:32

storage temp.

2-8°C

target post-translational modification

unmodified

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (whole molecule)-FITC antibody is specific for all mouse IgG subclasses. Affinity isolated antigen specific antibody is obtained from sheep anti-mouse IgG antiserum by immunospecific purification. The antibody preparation is then conjugated to Sigma Fluorescein Isothiocyanate (FITC), Isomer I.

Immunogen

Purified mouse IgG

Application

Anti-Mouse IgG (whole molecule)-FITC antibody may be used for immunofluorescent labeling of mouse spleen cells at a working antibody dilution of 1:32. For immunofluorescence of human ovarian adenocarcinoma cells stained with anti-integrin primary antibodies a working dilution of 1:50 was used. Immunolocalization of human spermatids was done using antibody dilution of 1:100. This antibody was also used for immunofluorescent tagging of leaf cells of Arabidopsis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
F0257F2012F9137
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

biological source

sheep

biological source

goat

biological source

goat

biological source

rabbit

technique(s)

direct immunofluorescence: 1:32

technique(s)

direct immunofluorescence: 1:32

technique(s)

direct immunofluorescence: 1:64 (minimum)

technique(s)

direct immunofluorescence: 1:100, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution


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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable



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Articles

In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents.


U I Ezeh et al.
Human reproduction (Oxford, England), 13(11), 3061-3065 (1998-12-16)
Limiting testicular biopsy for intracytoplasmic sperm injection (ICSI) to those with a high chance of having testicular spermatozoa has not been possible because of the poor predictive value of current clinical and laboratory methods. In order to predict testicular pathology
Fiona Ingrao et al.
Vaccines, 9(7) (2021-08-07)
Recombinant Newcastle disease viruses (rNDV) have been used as bivalent vectors for vaccination against multiple economically important avian pathogens. NDV-vectored vaccines expressing the immunogenic H5 hemagglutinin (rNDV-H5) are considered attractive candidates to protect poultry from both highly pathogenic avian influenza
Lénaïg Louboutin et al.
Journal of fish diseases, 45(5), 707-716 (2022-02-17)
Gilthead seabream represents a species of importance in Mediterranean aquaculture. The larval stage is particularly sensitive and frequently impacted in suboptimal environmental or sanitary conditions. In the present study, investigations were carried out in a seabream hatchery following an unusual



Global Trade Item Number

SKUGTIN
F6257-2ML04061837516979
F6257-1ML04061833618561
F6257-.5ML04061837516955

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