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Anti-Goat IgG (whole molecule)–FITC antibody produced in rabbit

Name: Anti-Goat IgG (whole molecule)–FITC antibody produced in rabbit
Brand: SIGMA
Price: 200 USD

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Rabbit Anti-Goat IgG (whole molecule)–Fluorescein isothiocyanate

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Pack Size	SKU	Availability	Price
1 mL		Check Cart for Availability	$200.00
2 mL		Check Cart for Availability	$335.00 $284.75

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162348

Conjugate:

FITC conjugate

Clone:

polyclonal

Application:

IF (d), IHC (p)

Citations:

$200.00

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Properties

biological source

rabbit

Quality Level

200

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:400, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

IgG antibodies are smallest and are found in all body fluids and capable of fighting bacterial and viral infection. It is the only type of antibody able to cross the placenta and immunise the foetus. The anti-goat IgG (whole molecule)-FITC antibody is useful in indirect immunofluorescence studies of immunised goat sera infected Trichostrongylus colubriformis (nematode). Antigens can be measured by quantitative immunofluorescence using image analysis, in tissue sections stained with FITC labelled rabbit anti goat IgG. Anti-goat IgG antibody reacts specifically with all goat immunoglobulins.

IgG is a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2.

Immunoglobulin G (IgG) is found in blood and has four polypeptide chains. It contains two identical 25 kDa κ or λ light (L) chains and two identical 50 kDa γ heavy (H) chains. The light and heavy chains are interconnected by disulfide bonds. It is the most important type of glycoprotein antibody present in human serum, secreted by the B cells. IgG exists in four isotypes: IgG1, IgG2, IgG3, and IgG4. IgG has the longest serum half-life compared to other immunoglobulins.

Immunogen

IgG purified from Goat

Application

Anti-Goat IgG (whole molecule)−FITC antibody has been used:

in double labeling for laminin and α-fetoprotein (AFP) at a dilution of 1:400
in immunohistochemistry at a dilution of 1:50
in immunocytochemistry
in immunochemical jagged-1 analyses
in immunofluorescence at a dilution of 1:200

Anti-goat IgG antibody can be used in antisera localization for neurofilament antigens obtained in rat peripheral nerve and can be done in tissues of rat and human peripheral and central nervous system by the aid of indirect immunofluorescence.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Immunoglobulin G (IgG) stimulates the classical pathway of the complement system. Maternal IgG is transferred to the fetus through the placenta. This is vital for the immune defense of the neonate against infections. IgG regulates antibody-dependent cell-mediated cytotoxicity (ADCC) and might be associated with allergy. It nullifies virus particles and toxins. Digestion of IgG by papain produces fragment antigen-binding (Fab). Pepsin digestion of IgG generates crystallizable (fc) fragment and a single dimeric F(ab)2. IgG levels in severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infected patients may be used as a possible tool to assess the severity and prognosis of coronavirus disease 2019 (COVID-19).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	F9887	F5387	F0382
Sigma-Aldrich F7367 Anti-Goat IgG (whole molecule)–FITC antibody produced in rabbit	Sigma-Aldrich F9887 Anti-Rabbit IgG (whole molecule)–FITC antibody produced in goat	Sigma-Aldrich F5387 Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat	Sigma-Aldrich F0382 Anti-Rabbit IgG (whole molecule)–FITC antibody produced in goat
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate
biological source rabbit	biological source goat	biological source goat	biological source goat
technique(s) direct immunofluorescence: 1:400, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400	technique(s) direct immunofluorescence: 1:160, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, indirect immunofluorescence: 1:32	technique(s) indirect immunofluorescence: 1:80
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution

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Storage Class

10 - Combustible liquids

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Global Trade Item Number

SKU	GTIN
F7367-.5ML	04061833619568
F7367-1ML	04061833619575
F7367-2ML	04061833619582

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