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Anti-Horse IgG (whole molecule)−FITC antibody produced in rabbit

Name: Anti-Horse IgG (whole molecule)−FITC antibody produced in rabbit
Brand: SIGMA
Price: 330.77 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Rabbit Anti-Horse IgG (whole molecule)−Fluorescein isothiocyanate

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2 mL	Available to ship TODAYfromMILWAUKEE	$341.00 $330.77

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00163518

Conjugate:

FITC conjugate

Clone:

polyclonal

Application:

IF (d)

Citations:

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Properties

biological source

rabbit

Quality Segment

200

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

direct immunofluorescence: suitable

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Horse IgG has different subclasses, IgG2a, IgG2b, IgG2c, IgG1 and IgG(T). Compared to other IgG molecules, IgG(T) has more carbohydrate content. In healthy individuals, total IgG is dispersed equally between circulating plasma and interstitial fluids.

Horse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in horse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.

Immunogen

Purified horse IgG

Application

Anti-Horse IgG (whole molecule) FITC antibody produced in rabbit has been used in indirect fluorescent antibody test. It has also been used as a secondary antibody to confirm equine arteritis virus (EAV).

Rabbit polyclonal anti-Horse IgG (whole molecule)−FITC antibody may be used to detect and quantitate the level of IgG in horse serum and biological fluids by fluorescent techniques. It may also be used as a secondary antibody in assays that use horse IgG as the primary antibody.

Biochem/physiol Actions

Anti-horse IgG (whole molecule)-FITC antibody is specific for horse IgG subclasses.

IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B- cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to the fetus through the placenta that is vital for immune defense of the neonate against infections.

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It mainly helps in immune defense.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	F7884	F5137	F8888
Sigma-Aldrich F7759 Anti-Horse IgG (whole molecule)−FITC antibody produced in rabbit	Sigma-Aldrich F7884 Anti-Dog IgG (whole molecule)–FITC antibody produced in rabbit	$Anti-Sheep IgG (whole molecule)−FITC antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich F5137 Anti-Sheep IgG (whole molecule)−FITC antibody produced in rabbit	Sigma-Aldrich F8888 Anti-Chicken IgY (IgG) (whole molecule)−FITC antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate	conjugate FITC conjugate
technique(s) direct immunofluorescence: suitable	technique(s) direct immunofluorescence: 1:32	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, indirect immunofluorescence: 1:100	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320 using human tonsil, direct immunofluorescence: 1:320 using human peripheral blood lymphocytes
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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