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F7879

Ferritin, cationized from horse spleen

10 mg/mL in 0.15 M NaCl

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2 mL
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$274.00

About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.26
MDL number:
Form:
liquid
Biological source:
horse spleen

$274.00


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biological source

horse spleen

Quality Segment

sterility

sterile-filtered

form

liquid

concentration

10 mg/mL in 0.15 M NaCl

technique(s)

cell culture | mammalian: suitable, electron microscopy: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

horse ... FTL(100051593)

General description

Ferritin, an iron-storage protein is usually present in the liver and spleen in mammals. Iron and ferritin are distributed relatively same in the eye. Ferritin is made up of heavy and light chains.

Application

A cationic derivative used to label negatively charged cell membranes for visualization by electron microscopy.
Ferritin is an iron storage protein that plays a central role in iron metabolism, and may be elevated in inflammatory or malignant diseases. It has been used in studies as an indicator of body iron supply, but may prove inaccurate in in the presence of chronic inflammation.
Ferritin, cationized from horse spleen has been used:
  • to incubate amoebae (106) in the study, to determine whether trophozoites are able to take up ferritin and internalise this protein for their growth in axenic culture
  • to determine phagosome-lysosome fusion by electron microscopy
  • to label freshly excised chick optic tecta in serum free incubation medium

Biochem/physiol Actions

Ferritin holds iron at its ferric state (Fe3+). The core of ferritin has the capability to hold 4500 iron molecules. It is essential to store iron in vertebrates. Ferritin is also essential to store and transport iron in invertebrates. It plays a key role in dietary iron absorption.

Preparation Note

Prepared by coupling horse spleen ferritin with N,N-dimethyl-1,3-propanediamine (DMPA).

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This Item
F6879F4503178440
description

10 mg/mL in 0.15 M NaCl

description

Type V, 10 μg/mL in 0.15 M NaCl, 10 mM Tris, pH 8.0, containing 0.1% sodium azide

description

Type I, saline solution

description

Ferritin, Apo-, Equine Spleen, CAS 9013-31-4, is a native protein shell of ferritin molecule lacking iron.

biological source

horse spleen

biological source

human spleen

biological source

-

biological source

-

technique(s)

electron microscopy: suitable, cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

concentration

10 mg/mL in 0.15 M NaCl

concentration

10 μg/mL in 0.15 M NaCl, 10 mM Tris, pH 8.0, containing 0.1% sodium azide

concentration

-

concentration

-

form

liquid

form

liquid

form

saline solution

form

lyophilized solid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Gene Information

horse ... FTL(100051593)

Gene Information

human ... FTH1(2495), FTL(2512)

Gene Information

-

Gene Information

-


Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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