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F4503

Sigma-Aldrich

Ferritin from equine spleen

Type I, saline solution

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CAS Number:
9007-73-2
MDL number:
MFCD00081593
NACRES:
NA.77

Quality Level

200

sterility

sterile-filtered

type

Type I

form

saline solution

mol wt

H subunit ~21 kDa
L subunit ~19 kDa

color

red to brown

cation traces

Cd: ≤1% (as % of ferritin)

storage temp.

2-8°C

General description

A shell of 24 protein subunits (apoferritin) and a core Fe 3+ ions with a peptide MW of 440 kDa. Review of the ferritin-like superfamily of iron-storage proteins, virtually ubiquitous in life on earth.

Application

Ferritin from equine spleen has been used:
  • in mycobacterial growth assay
  • in size-exclusion chromatography
  • in labeling of macrophage cells
  • as a reference standard in muon spin spectroscopy

Biochem/physiol Actions

A ubiquitous iron storage protein that plays a key role in iron metabolism. It serves as an intracellular iron reserve (particularly in spleen, liver, intestinal mucosa, and bone marrow) and functions in iron detoxification. Studies have shown that ferritin iron incorporation is mediated by a ferroxidase activity associated with ferritin H subunits and a nucleation center associated with ferritin L subunits. Release of iron from ferritin has an essential role in iron-dependent lipid peroxidation and may contribute to free radical-induced cell damage in vivo. Therefore, by binding iron, ferritin may function as an antioxidant.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the concentration of ferritin in solutions of Product F4503, Ferritin from equine spleen?

    The concentration of ferritin is lot specific and can be found on the Certificate of Analysis (CofA) for the specific lot.

  4. Has Product F4503, Ferritin from equine spleen, been cell culture tested?

    The solution is sterile-filtered but has not been tested in cell culture.

  5. Where is iron located in the ferritin molecule of Product F4503, Ferritin from equine spleen?

    Iron is located in the core of the ferritin molecule consisting of 24 subunits. Up to 4500 iron ions can be contained in this core.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Adipocyte model of Mycobacterium tuberculosis infection reveals differential iron availability to bacilli in the lipid rich caseous environment
Nandy A, et al.
Infection and Immunity, IAI-00041 (2018)
Sella Kim et al.
Journal of molecular biology, 428(24 Pt B), 5007-5018 (2016-11-03)
Iron and oxygen chemistry is mediated by iron proteins for many biological functions. Carboxylate-bridged diiron enzymes including ferritin have the common mechanism of oxygen activation via peroxodiferric intermediates. However, the route for iron uptake and the structural identification of intermediates...
Shi Qiu et al.
Ultramicroscopy, 216, 113036-113036 (2020-06-17)
A new method for imaging liquid specimens with atom probe tomography (APT) is proposed by introducing graphene encapsulation. By tuning the encapsulation speed and the number of encapsulations, controllable volumes of liquid can be encapsulated on a pre-sharpened specimen tip...
Defining the Architecture of the Core Machinery for the Assembly of Fe-S Clusters in Human Mitochondria
Methods in Enzymology, 595, 107-160 (2017)
Italo Moglia et al.
Journal of inorganic biochemistry, 183, 184-190 (2017-12-28)
Ferritin is a globular protein that consists of 24 subunits forming a hollow nanocage structure that naturally stores iron oxyhydroxides. Elimination of iron atoms to obtain the empty protein called apoferritin is the first step to use this organic shell...
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