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FD20S

Isotiocianato de fluoresceína-dextrano

average mol wt 20,000

Sinónimos:

FITC–Dextrano

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A ustedes/SKUDisponibilidadPrecio
100 mg

Disponible para enviar HOYdesdeMILWAUKEE

$174.00
250 mg

Disponible para enviar HOYdesdeMILWAUKEE

$350.00
1 g

Disponible para enviar HOYdesdeMILWAUKEE

$1,170.00
$877.50

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Número CAS:
60842-46-8
UNSPSC Code:
12352201
NACRES:
NA.25
MDL number:
MFCD00131092

$174.00

Disponible para enviar HOYDetalles

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biological source

sugar beets root

Quality Segment

200

conjugate

FITC conjugate

form

powder

mol wt

average mol wt 20,000

extent of labeling

0.003-0.020 mol FITC per mol glucose

color

yellow to dark yellow

solubility

water: 25 mg/mL

storage temp.

2-8°C

General description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

Dextrano marcado con isotiocianato de fluoresceína para su posible uso en estudios de perfusión en animales.
FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.
FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination.[1] Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. Fluorescein-dextran, 20 kDa, may be used to study cell processes such as endocytosis and osmoporation.[2] FITC-dextran-20 may be used to study endothelial or epithelial monolayer porosity, apical-basolateral movements within cell layers and cell junction permeability. FITC-dextran-20 may be used as a model compound to study drug release from structures such as hydrogens and collagen minirods.[3]

Preparation Note

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Other Notes

Similar to FD-20, but produced by Sigma.
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

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Este artículo
FD20FD2000SFD4
Isotiocianato de fluoresceína-dextrano average mol wt 20,000

Sigma-Aldrich

FD20S

Isotiocianato de fluoresceína-dextrano

Isotiocianato de fluoresceína-dextrano average mol wt 20,000

Sigma-Aldrich

FD20

Isotiocianato de fluoresceína-dextrano

Isotiocianato de fluoresceína-dextrano average mol wt 2,000,000

Sigma-Aldrich

FD2000S

Isotiocianato de fluoresceína-dextrano

Isotiocianato de fluoresceína-dextrano average mol wt 3,000-5,000

Sigma-Aldrich

FD4

Isotiocianato de fluoresceína-dextrano

biological source

sugar beets root

biological source

bacterial (Leuconostoc mesenteroides)

biological source

synthetic (organic)

biological source

synthetic

solubility

water: 25 mg/mL

solubility

water: 25 mg/mL

solubility

H2O: soluble 25 mg/mL

solubility

H2O: 25 mg/mL

form

powder

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

color

yellow to dark yellow

color

light yellow to dark orange

color

yellow to orange

color

light yellow to dark orange

extent of labeling

0.003-0.020 mol FITC per mol glucose

extent of labeling

0.001-0.020 mol FITC per mol glucose

extent of labeling

0.003-0.020 mol FITC per mol glucose

extent of labeling

0.001-0.020 mol FITC per mol glucose

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pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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