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FD500S

Fluorescein isothiocyanate–dextran

average mol wt 500,000

Synonym(s):

FITC–Dextran

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Size/SKUAvailabilityPrice
100 mg
Please contact Customer Service for Availability
$127.00
250 mg
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$250.00
1 g
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$755.00
5 g
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$2,430.00

About This Item

CAS Number:
60842-46-8
UNSPSC Code:
12352201
NACRES:
NA.25
MDL number:
MFCD00131092

$127.00

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biological source

synthetic (organic)

Quality Segment

conjugate

FITC conjugate

form

powder

mol wt

average mol wt 500,000

extent of labeling

0.003-0.020 mol FITC per mol glucose

color

yellow to orange

solubility

H2O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange

storage temp.

2-8°C

General description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

FITC-dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Small FITC-dextrans are also used to measure processes such as endocytosis and cell junction permeability. Fluorescein-dextran, 500 kDa, may be used as a cerebral spinal fluid (CSF) tracer. FITC-dextran-500 may be used to study endothelial or epithelial monolayer porosity, apical-basolateral movements within cell layers and cell junction permeability.
Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.

Preparation Note

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Other Notes

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

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Fluorescein isothiocyanate–dextran average mol wt 500,000

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FD500S

Fluorescein isothiocyanate–dextran

Fluorescein isothiocyanate–dextran average mol wt 2,000,000

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FD2000S

Fluorescein isothiocyanate–dextran

Fluorescein isothiocyanate–dextran average mol wt 40,000

Sigma-Aldrich

FD40S

Fluorescein isothiocyanate–dextran

Sigma-Aldrich

Sigma-Aldrich

46945

Fluorescein isothiocyanate–dextran

description

average mol wt 500,000

description

average mol wt 2,000,000

description

average mol wt 40,000

description

average mol wt 70,000, (FITC:Glucose = 1:250)

biological source

synthetic (organic)

biological source

synthetic (organic)

biological source

synthetic (organic)

biological source

synthetic

solubility

H2O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange

solubility

H2O: soluble 25 mg/mL

solubility

H2O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange

solubility

H2O: 50 mg/mL, hazy, orange to very deep orange

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

form

powder

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

color

yellow to orange

color

yellow to orange

color

yellow to dark orange

color

light yellow to very dark yellow, light orange to very dark orange

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Codes

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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