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Guanosine 5′-[β,γ-imido]triphosphate trisodium salt hydrate

Name: Guanosine 5&#8242;-[&#946;,&#947;-imido]triphosphate trisodium salt hydrate
Brand: SIGMA
Price: 196 USD
Availability: InStock

≥85% (HPLC), synthetic (organic), powder

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Synonym(s):

β:γ-Imidoguanosine 5′-triphosphate trisodium salt hydrate, 5′-Guanylyl-imidodiphosphate trisodium salt hydrate, GMP-PNP, Gpp(NH)p

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Size/SKU	Availability	Price
5 mg	Check Cart for Availability	$196.00
25 mg	Check Cart for Availability	$723.00
100 mg	Check Cart for Availability	$2,200.00

About This Item

Empirical Formula (Hill Notation):

C₁₀H₁₄N₆Na₃O₁₃P₃ · xH₂O

CAS Number:

148892-91-5

Molecular Weight:

588.14 (anhydrous basis)

UNSPSC Code:

41106305

PubChem Substance ID:

24895020

NACRES:

NA.51

MDL number:

MFCD00084770

Assay:

≥85% (HPLC)

Biological source:

synthetic (organic)

Form:

powder

Solubility:

H₂O: 50 mg/mL

Storage temp.:

−70°C

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Properties

Product Name

Guanosine 5′-[β,γ-imido]triphosphate trisodium salt hydrate, ≥85% (HPLC), powder

biological source

synthetic (organic)

Quality Segment

200

assay

≥85% (HPLC)

form

powder

color

white

solubility

H₂O: 50 mg/mL

shipped in

dry ice

storage temp.

−70°C

SMILES string

[Na+].[Na+].[Na+].Nc1nc(O)c2ncn([C@@H]3O[C@H](COP([O-])(=O)OP([O-])(=O)NP(O)([O-])=O)[C@@H](O)[C@H]3O)c2n1

InChI

1S/C10H17N6O13P3.3Na/c11-10-13-7-4(8(19)14-10)12-2-16(7)9-6(18)5(17)3(28-9)1-27-32(25,26)29-31(23,24)15-30(20,21)22;;;/h2-3,5-6,9,17-18H,1H2,(H,25,26)(H3,11,13,14,19)(H4,15,20,21,22,23,24);;;/q;3*+1/p-3/t3-,5-,6-,9-;;;/m1.../s1

InChI key

QGJNRMUNXAROIT-CYCLDIHTSA-K

Description

General description

Guanosine 5′-[β,γ-imido]triphosphate (GMP-PNP, Gpp(NH)p) is a non-hydrolyzable analog of GTP that binds and irreversibly activates G proteins. Gpp(NH)p is used in a variety of applications that involve GTP binding, including GTP-activation, GTP-inhibition, GTP transport, GTP hydrolysis, and GTP structure stabilization. Since a cycle of GTP binding, hydrolysis, and release is required for the initiation of protein translocation across the endoplasmic reticulum, guanosine 5′-[β,γ-imido]triphosphate is often used in studies of protein synthesis. Gpp(NH)p is used to activate ADP-ribosylation factors and to modulate G proteins involved in cell signaling, protein synthesis and other metabolic processes.

Application

Non-hydrolyzable analog of GTP; the preferred GTP analog to activate ADP-ribosylation factor.

Guanosine 5′-[β,γ-imido]triphosphate trisodium salt hydrate has been used: to study K-RAS interactions with the membrane, for the crystallization of Mycobacterium tuberculosis FtsZ, as a translation initiation inhibitor for the analysis of the 48S pre-initiation complex.

Biochem/physiol Actions

Guanosine 5′-[β,γ-imido]triphosphate binds and irreversibly activates G proteins. Since a cycle of GTP binding, hydrolysis, and release is required for the initiation of protein translocation across the endoplasmic reticulum, this non-hydrolyzable GTP analog is often used in studies of protein synthesis.

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This Item	G9002	51060	M3509
Sigma-Aldrich G0635 Guanosine 5′-[β,γ-imido]triphosphate trisodium salt hydrate	Sigma-Aldrich G9002 Guanosine 5′-triphosphate tris salt	Sigma-Aldrich 51060 Guanosine 5′-diphosphate disodium salt	Sigma-Aldrich M3509 β,γ-Methyleneguanosine 5′-triphosphate sodium salt
description ≥85% (HPLC), powder	description ≥93% (HPLC), powder	description ≥90% (HPLC)	description ≥98% (HPLC)
biological source synthetic (organic)	biological source bovine (casein), bacterial (corynebacterium sp), yeast, Porcine brain	biological source synthetic, Porcine brain, microbial (Corynebacterium sp)	biological source synthetic (organic)
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 200
form powder	form powder	form powder	form powder
assay ≥85% (HPLC)	assay ≥93% (HPLC)	assay ≥90% (HPLC)	assay ≥98% (HPLC)
solubility H₂O: 50 mg/mL	solubility H₂O: soluble 50 mg/mL, clear to slightly hazy, colorless	solubility H₂O: 50 mg/mL, clear, colorless	solubility water: 50 mg/mL, clear, colorless
storage temp. −70°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C