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Granulocyte-Macrophage Colony-Stimulating Factor from rat

Name: Granulocyte-Macrophage Colony-Stimulating Factor from rat
Brand: SIGMA

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophililzed powder

Synonym(s):

GM-CSF

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About This Item

MDL number:

MFCD01868356

NACRES:

NA.77

UNSPSC Code:

12352202

Biological source:

rat

Recombinant:

expressed in E. coli

Assay:

>97% (SDS-PAGE)

Form:

lyophilized powder

Mol wt:

14.7 kDa

Impurities:

<1 EU/μg

Pricing and availability is not currently available.

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Properties

Product Name

Granulocyte-Macrophage Colony-Stimulating Factor from rat, GM-CSF, recombinant, expressed in E. coli

biological source

rat

Quality Segment

100

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.03-0.1 ng/mL

quality

endotoxin tested

mol wt

14.7 kDa

packaging

pkg of 5X5 μg, pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

<1 EU/μg

UniProt accession no.

P48750

storage temp.

−20°C

Gene Information

rat ... CSF2(116630), Csf2(116630)

Description

Biochem/physiol Actions

Four distinct colony-stimulating factors (CSFs) promoting survival, proliferation and differentiation of bone marrow precursor cells have been well characterized: granulocyte-macrophage-CSF (GM-CSF), granulocyte-CSF (G-CSF), macrophage-CSF (M-CSF), and interleukin-3 (IL-3, Multi-CSF). GM-CSF is a multipotential growth factor, stimulating proliferation of progenitor cells from more than one hematopoietic lineage (granulocyte, macrophage, and eosinophil). GM-CSF stimulates colony formation from pluripotent progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. GM-CSF induces myeloid progenitor cells from bone marrow to form colonies containing macrophages and granulocytes in a semisolid media. It is produced by various cell types (activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts) in response to cytokine and inflammatory stimuli. In addition to granulocyte-macrophage progenitors, GMCSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF can induce nonhematopoietic cells such as endothelial cells to migrate and proliferate. It also stimulates the proliferation of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity GM-CSF receptors contain a GM-CSF-specific α?chain and a common α ?chain.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.

Preparation Note

Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is produced from a DNA sequence encoding mature rat GM-CSF. This recombinant protein is a mixture of two rat GM-CSFs, the 127 amino acid form and the 128 amino acid methionyl form. Mature rat GM-CSF has a calculated mass of 14.7 kDa. GM-CSF, an acidic glycoprotein, is species-specific.

Analysis Note

The biological activity is measured by its ability to induce proliferation of the factor-dependent mouse cell line, DA3.

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This Item	SRP3050	H5666	SRP3201
Sigma-Aldrich G0792 Granulocyte-Macrophage Colony-Stimulating Factor from rat	Sigma-Aldrich SRP3050 Granulocyte-Macrophage Colony-Stimulating Factor human	Sigma-Aldrich H5666 Granulocyte-Macrophage Colony-Stimulating Factor human	Sigma-Aldrich SRP3201 GM-CSF from mouse
description GM-CSF, recombinant, expressed in E. coli	description GM-CSF, Animal-component free, recombinant, expressed in E. coli, suitable for cell culture	description GM-CSF, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture	description Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
assay >97% (SDS-PAGE)	assay ≥98% (SDS-PAGE)	assay ≥95% (SDS-PAGE)	assay ≥98% (HPLC), ≥98% (SDS-PAGE)
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in HEK 293 cells	recombinant expressed in E. coli
biological source rat	biological source human	biological source human	biological source mouse
Quality Level 100	Quality Level 200	Quality Level 100	Quality Level -
form lyophilized powder	form lyophilized	form lyophilized powder	form lyophilized
impurities <1 EU/μg	impurities ≤1 EU/μg endotoxin, tested	impurities ≤1 EU/mg	impurities <0.1 EU/μg endotoxin, tested

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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