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G0792

Granulocyte-Macrophage Colony-Stimulating Factor from rat

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophililzed powder

Synonym(s):

GM-CSF

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5 μg

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$617.00
$524.45

About This Item

MDL number:
MFCD01868356
NACRES:
NA.77
UNSPSC Code:
12352202
Biological source:
rat
Recombinant:
expressed in E. coli
Assay:
>97% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
14.7 kDa
Impurities:
<1 EU/μg

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Product Name

Granulocyte-Macrophage Colony-Stimulating Factor from rat, GM-CSF, recombinant, expressed in E. coli

biological source

rat

Quality Segment

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.03-0.1 ng/mL

quality

endotoxin tested

mol wt

14.7 kDa

packaging

pkg of 5X5 μg, pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

<1 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

Biochem/physiol Actions

Four distinct colony-stimulating factors (CSFs) promoting survival, proliferation and differentiation of bone marrow precursor cells have been well characterized: granulocyte-macrophage-CSF (GM-CSF), granulocyte-CSF (G-CSF), macrophage-CSF (M-CSF), and interleukin-3 (IL-3, Multi-CSF). GM-CSF is a multipotential growth factor, stimulating proliferation of progenitor cells from more than one hematopoietic lineage (granulocyte, macrophage, and eosinophil). GM-CSF stimulates colony formation from pluripotent progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. GM-CSF induces myeloid progenitor cells from bone marrow to form colonies containing macrophages and granulocytes in a semisolid media. It is produced by various cell types (activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts) in response to cytokine and inflammatory stimuli. In addition to granulocyte-macrophage progenitors, GMCSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF can induce nonhematopoietic cells such as endothelial cells to migrate and proliferate. It also stimulates the proliferation of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity GM-CSF receptors contain a GM-CSF-specific α?chain and a common α ?chain.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.

Preparation Note

Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is produced from a DNA sequence encoding mature rat GM-CSF. This recombinant protein is a mixture of two rat GM-CSFs, the 127 amino acid form and the 128 amino acid methionyl form. Mature rat GM-CSF has a calculated mass of 14.7 kDa. GM-CSF, an acidic glycoprotein, is species-specific.

Analysis Note

The biological activity is measured by its ability to induce proliferation of the factor-dependent mouse cell line, DA3.

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Granulocyte-Macrophage Colony-Stimulating Factor from rat GM-CSF, recombinant, expressed in E. coli

Sigma-Aldrich

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Granulocyte-Macrophage Colony-Stimulating Factor human

Granulocyte-Macrophage Colony-Stimulating Factor human GM-CSF, Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

Sigma-Aldrich

SRP3050

Granulocyte-Macrophage Colony-Stimulating Factor human

description

GM-CSF, recombinant, expressed in E. coli

description

GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

description

GM-CSF, recombinant, expressed in E. coli, suitable for cell culture

description

GM-CSF, Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

biological source

rat

biological source

mouse

biological source

human

biological source

human

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized

mol wt

14.7 kDa

mol wt

~15 kDa (125 amino acids including N-terminal methionine)

mol wt

14.6 kDa

mol wt

14.6 kDa

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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