G10120
Sephadex® G-10
Medium
Synonym(s):
Gel filtration resin, Sephadex G-10 Medium
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| Size/SKU | Availability | Price |
|---|---|---|
10 g | Check Cart for Availability | $109.00 |
50 g | Check Cart for Availability | $302.00 |
About This Item
$109.00
Check Cart for Availability
Quality Segment
technique(s)
protein array: suitable
matrix active group
phase
swelling
1 g swells to 2-3 mL
application(s)
life science and biopharma
compatibility
Cytiva
General description
Sephadex G-10 is a well-established gel filtration resin specifically designed for desalting and buffer exchange of peptides and small biomolecules. It boasts the smallest exclusion limit within the Sephadex range, making it highly effective in these applications. Sephadex itself is a gel filtration resin that is created through the crosslinking of dextran with epichlorohydrin. The various types of Sephadex exhibit different degrees of cross-linking, leading to variations in swelling properties and molecular fractionation range. Sephadex G-10 falls into the category of G-types, alongside four other types, ranging from G-10 for small molecules to G-75 for larger molecules.
Application
Sephadex® G-10 has been used for the reconstitution of membrane proteins into a giant proteoliposome.
Sephadex® G-10 has been used as a size exclusion material for the separation of the conjugated N-glycans from the surplus dye. It has also been used in desalting for reducing salt concentration for anion-exchange (AEx), cation-exchange (CEx) chromatography.
Biochem/physiol Actions
Sephadex G-10 serves as a gel filtration medium for gel filtration chromatography and protein chromatography. Its versatility is demonstrated by its applications in cancer studies and its ability to determine fluoride levels in diverse samples, including tap water, river water, and green tea samples.
Features and Benefits
- Desalts, removes contaminants, and transfers to a new buffer in one step.
- Suitable for buffer exchange and cleanup of biological samples,
- Effective for peptides, small proteins, and oligosaccharides with a molecular weight larger than 700.
- Efficiently removes salts, dyes, and radioactive labels.
- Lowest exclusion limit of 700 molecular weight in the Sephadex G-range
Legal Information
Sephadex is a registered trademark of Cytiva
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This Item | |||
|---|---|---|---|
| description Medium | description Superfine | description Medium | description BioReagent, DNA grade, Molecular Biology, fine |
| application(s) life science and biopharma | application(s) life science and biopharma | application(s) - | application(s) - |
| matrix active group phase | matrix active group phase | matrix active group polymer | matrix active group - |
| compatibility Cytiva | compatibility Cytiva | compatibility - | compatibility - |
| technique(s) protein array: suitable | technique(s) buffer exchange: suitable | technique(s) LPLC: suitable | technique(s) DNA purification: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| swelling 1 g swells to 2-3 mL | swelling 1 g swells to 12-15 mL | swelling -1 g swells to 9-11 mL | swelling 1 g swells to 9-11 mL |
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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