G10120

Sephadex^® G-10

Medium

• Synonym(s): Gel filtration resin, Sephadex G-10 Medium
• CAS Number: 9050-68-4
• MDL number: MFCD00002744
• UNSPSC Code: 23151817
• NACRES: NA.56

Properties

• Quality Level: 200
• technique(s): protein array: suitable
• matrix active group: phase
• swelling: 1 g swells to 2-3 mL
• application(s): life science and biopharma
• compatibility: Cytiva

Description

General description

Sephadex G-10 is a well-established gel filtration resin specifically designed for desalting and buffer exchange of peptides and small biomolecules. It boasts the smallest exclusion limit within the Sephadex range, making it highly effective in these applications. Sephadex itself is a gel filtration resin that is created through the crosslinking of dextran with epichlorohydrin. The various types of Sephadex exhibit different degrees of cross-linking, leading to variations in swelling properties and molecular fractionation range. Sephadex G-10 falls into the category of G-types, alongside four other types, ranging from G-10 for small molecules to G-75 for larger molecules.

Application

Sephadex^® G-10 has been used as a size exclusion material for the separation of the conjugated N-glycans from the surplus dye.[1] It has also been used in desalting for reducing salt concentration for anion-exchange (AEx), cation-exchange (CEx) chromatography.[2]

Sephadex^® G-10 has been used for the reconstitution of membrane proteins into a giant proteoliposome.[3]

Biochem/physiol Actions

Sephadex G-10 serves as a gel filtration medium for gel filtration chromatography and protein chromatography. Its versatility is demonstrated by its applications in cancer studies and its ability to determine fluoride levels in diverse samples, including tap water, river water, and green tea samples.

Features and Benefits

• Desalts, removes contaminants, and transfers to a new buffer in one step.
• Suitable for buffer exchange and cleanup of biological samples,
• Effective for peptides, small proteins, and oligosaccharides with a molecular weight larger than 700.
• Efficiently removes salts, dyes, and radioactive labels.
• Lowest exclusion limit of 700 molecular weight in the Sephadex G-range

Legal Information

Sephadex is a registered trademark of Cytiva

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)