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O-Glycosidase from Streptococcus pneumoniae

Name: O-Glycosidase from <I>Streptococcus pneumoniae</I>
Brand: SIGMA

recombinant, expressed in E. coli, buffered aqueous solution

Synonym(s):

Endo-α-N-acetylgalactosaminidase, O-Glycanase

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About This Item

CAS Number:

9032-92-2

EC Number:

3.2.1.97 (BRENDA, IUBMB)

UNSPSC Code:

12352204

EC Number:

232-888-2

NACRES:

NA.32

MDL number:

MFCD12922510

Recombinant:

expressed in E. coli

Concentration:

≥800 units/mL

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Properties

recombinant

expressed in E. coli

Quality Segment

200

conjugate

(O-linked)

form

buffered aqueous solution

mol wt

180 kDa

concentration

≥800 units/mL

shipped in

wet ice

storage temp.

2-8°C

Description

Biochem/physiol Actions

Releases unsubstituted Ser- and Thr-linked β-Gal-(1→3)-α-GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent the liberation of the oligosaccharide from the protein. Pretreament with glycolytic enzymes to remove substituent saccharides from the O-glycan may be needed prior to cleavage using O-glycosidase..

Packaging

Supplied with 5× Reaction Buffer, 250 mM NaH₂PO₄ pH 5.0.

Physical form

Solution in 50 mM sodium phosphate, pH 7.5

Analysis Note

Screened for presence of: β-galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, neuraminidase, and proteases. See Certificate of Analysis for lot specific information.

Other Notes

One unit will hydrolyze 1 μmole of p-nitrophenyl galacto-N-bioside (β-Gal-(1→3)-α-GalNAc-1→ΟC₆H₄NO₂) per min at 37 °C at pH 6.5.

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This Item	A6805	N7271	E0639
Sigma-Aldrich G1163 O-Glycosidase from Streptococcus pneumoniae	Sigma-Aldrich A6805 β-N-Acetylglucosaminidase from Streptococcus pneumoniae	Sigma-Aldrich N7271 α(2→3) Neuraminidase from Streptococcus pneumoniae	Sigma-Aldrich E0639 Endoglycosidase F2 from Elizabethkingia miricola
description recombinant, expressed in E. coli, buffered aqueous solution	description recombinant, expressed in E. coli, buffered aqueous solution	description buffered aqueous solution	description recombinant, expressed in E. coli, 20 U/mg
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant -	recombinant expressed in E. coli
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form solution
concentration ≥800 units/mL	concentration -	concentration ≥5 units/mL	concentration -
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
mol wt 180 kDa	mol wt -	mol wt -	mol wt 32 kDa

Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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