Autoclaving is possible for this product when in swollen (wetted) form. Autoclave at 121 °C for up to 30 minutes at neutral pH; chromatographic performance remains unaffected. Autoclaving in the dry state is not recommended, as heating dry resin above about 120 °C may degrade the dextran matrix.
G25150
Sephadex® G-25
Medium
Synonym(s):
Sephadex G-25 Medium, Sephadex® G-25 resins, Size exclusion resins
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| Size/SKU | Availability | Price |
|---|---|---|
10 g | Check Cart for Availability | $161.00 |
50 g | Check Cart for Availability | $530.00 |
About This Item
Quality Segment
technique(s)
buffer exchange: suitable
matrix active group
phase
swelling
1 g swells to 4-6 mL
bead size
50-150 μm
application(s)
life science and biopharma
compatibility
Cytiva
SMILES string
O1[C@H]([C@@H]([C@@H]([C@@H]([C@H]1CO)O)O)O)O.OC(CO)CO
InChI
1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1
InChI key
BCPVZFFJGCURNR-OCOFDJSDSA-N
General description
Sephadex® G-25 Medium is a size exclusion chromatography or gel filtration resin that is created by crosslinking dextran with epichlorohydrin. The various types of Sephadex can differ in their degree of cross-linking, resulting in variances in their swelling capacity and molecular fractionation range. Sephadex G-25 is one option available among the different G-types of Sephadex. Sephadex G-25 belongs to a range of five G-types, catering to different molecule sizes. Specifically, G-10 is suitable for small molecules, while G-75 is designed to accommodate larger molecules. Sephadex G-25 provides industrial requirements by ensuring a dependable supply and offering extensive technical and regulatory assistance.
Application
Fractionation Range (MW)
Dextrans: 100 - 5,000
Globular Proteins 1,000 - 5,000
Sephadex® G-25 has been used:
Dextrans: 100 - 5,000
Globular Proteins 1,000 - 5,000
Sephadex® G-25 has been used:
Sephadex® G-25 has been used to separate the labelled proteins from unreacted dye N-hydroxysuccinimide (NHS) esters by gel permeation chromatography.
Biochem/physiol Actions
Sephadex® G-25 is a well-established gel filtration resin, widely used for desalting and buffer exchange in industrial applications. Its hydrophilic matrix minimizes nonspecific adsorption, resulting in high recoveries during these processes for proteins and nucleic acids. Sephadex® G-25 is usually used in affinity chromatography, protein chromatography and gel filtration chromatography.
Features and Benefits
- Rapid desalting, contaminant removal, and transfer to a new buffer in a single step.
- High recovery rate with minimal sample dilution.
- Offered in prepacked HiPrep Desalting and HiTrap desalting columns for convenient and fast desalting.
- Specifically designed as a BioProcess resin for industrial applications.
Legal Information
Sephadex is a registered trademark of Cytiva
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This Item | |||
|---|---|---|---|
| description Medium | description Coarse | description BioReagent, DNA grade, Molecular Biology, Superfine | description Fine |
| compatibility Cytiva | compatibility - | compatibility Cytiva | compatibility Cytiva |
| matrix active group phase | matrix active group - | matrix active group phase | matrix active group phase |
| technique(s) buffer exchange: suitable | technique(s) LPLC: suitable | technique(s) buffer exchange: suitable | technique(s) LPLC: suitable, buffer exchange: suitable |
| application(s) life science and biopharma | application(s) - | application(s) life science and biopharma | application(s) life science and biopharma |
| swelling 1 g swells to 4-6 mL | swelling 1 g swells to 4-6 mL gel | swelling - | swelling 1 g swells to 4-6 mL |
| Quality Level 200 | Quality Level 200, 100 | Quality Level 200 | Quality Level 200 |
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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