Skip to Content
MilliporeSigma

S5772

Sephadex® G-25

BioReagent, DNA grade, Molecular Biology, Superfine

Slide 1 of 2
Sign In to View Organizational & Contract Pricing.

Select a Size

25 G

$305.00

10 G

$267.75

$305.00

Available to ship TODAYDetails

About This Item

CAS Number:
9041-35-4
UNSPSC Code:
23151817
NACRES:
NA.52
MDL number:
MFCD00132770

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Sephadex® G-25, BioReagent, DNA grade, Molecular Biology, Superfine

SMILES string

O1[C@H]([C@@H]([C@@H]([C@@H]([C@H]1CO)O)O)O)O.OC(CO)CO

InChI

1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1

InChI key

BCPVZFFJGCURNR-OCOFDJSDSA-N

grade

Molecular Biology

product line

BioReagent

form

beads

technique(s)

buffer exchange: suitable

matrix active group

phase

bead diameter

20-50 μm

pore size

exclusion limit (10 bp dsDNA; (rA)10)

pH range

2-13

application(s)

life science and biopharma

compatibility

Cytiva

foreign activity

DNase, RNase, none detected

storage temp.

room temp

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
G25300G2580G25150
Sephadex® G-25 BioReagent, DNA grade, Molecular Biology, Superfine

Sigma-Aldrich

S5772

Sephadex® G-25

Sephadex® G-25 Coarse

Sigma-Aldrich

G25300

Sephadex® G-25

Sephadex® G-25 Fine

Sigma-Aldrich

G2580

Sephadex® G-25

Sephadex® G-25 Medium

Sigma-Aldrich

G25150

Sephadex® G-25

technique(s)

buffer exchange: suitable

technique(s)

LPLC: suitable

technique(s)

buffer exchange: suitable

technique(s)

buffer exchange: suitable

compatibility

Cytiva

compatibility

-

compatibility

Cytiva

compatibility

Cytiva

matrix active group

phase

matrix active group

-

matrix active group

phase

matrix active group

phase

application(s)

life science and biopharma

application(s)

-

application(s)

life science and biopharma

application(s)

life science and biopharma

grade

DNA grade

grade

-

grade

-

grade

-

product line

BioReagent

product line

-

product line

-

product line

-

Application

Sephadex® G-25 is suitable for buffer exchange and desalting oligonucleotides for use in sequencing, PCR, and labeling applications. It was used to isolate two different peptides of Pronase E extracted from Alaska Pollack skin.[1]

Features and Benefits

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

General description

Sephadex® G-25, Superfine is an often-cited gel filtration medium for desalting and buffer exchange. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Superfine” indicates the smallest particle size available.

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000465186
0000465186
0000376986
0000353084
0000351225

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

S K Kim et al.
Journal of agricultural and food chemistry, 49(4), 1984-1989 (2001-04-20)
Gelatin extracted from Alaska pollack skin was hydrolyzed with serial digestions in the order of Alcalase, Pronase E, and collagenase using a three-step recycling membrane reactor. The fraction from the second step, which was hydrolyzed with Pronase E, was composed
Kelsey E Knewtson et al.
ACS omega, 4(7), 12955-12968 (2019-08-29)
Antibody-drug conjugates are an important class of cancer therapeutics. These agents generally bind a specific cell surface receptor, undergo receptor-mediated endocytosis, and enter the endosomal-lysosomal system, where the environment in these organelles facilitates the release of a membrane-permeable cytotoxin. By
Veronica Murta et al.
Journal of neurochemistry, 144(6), 748-760 (2017-12-28)
Secondary neuronal death is a serious stroke complication. This process is facilitated by the conversion of glial cells to the reactive pro-inflammatory phenotype that induces neurodegeneration. Therefore, regulation of glial activation is a compelling strategy to reduce brain damage after
Veronika Horkova et al.
Cell reports, 30(5), 1504-1514 (2020-02-06)
Overtly self-reactive T cells are removed during thymic selection. However, it has been recently established that T cell self-reactivity promotes protective immune responses. Apparently, the level of self-reactivity of mature T cells must be tightly balanced. Our mathematical model and experimental data
Nerine T Joseph et al.
The Journal of endocrinology, 202(1), 179-190 (2009-04-22)
Two GnRH isoforms (cGnRH-I and GnRH-II) and two GnRH receptor subtypes (cGnRH-R-I and cGnRH-R-III) occur in chickens. Differential roles for these molecules in regulating gonadotrophin secretion or other functions are unclear. To investigate this we cloned cGnRH-R-III from a broiler
View All Related Papers

Related Content

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service