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G2626

L-Glutamic Dehydrogenase from bovine liver

Type II, 50% glycerol solution, ≥35 units/mg protein

Synonym(s):

L-GLDH, L-Glutamate:NAD[P]+ Oxidoreductase (deaminating), Glutamate Dehydrogenase from bovine liver

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Size/SKUAvailabilityPrice
20 mg
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$107.00
50 mg
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$190.00
100 mg
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$298.00
500 mg
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$1,020.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
≥35 units/mg protein
Biological source:
bovine liver

$107.00


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biological source

bovine liver

Quality Segment

type

Type II

form

glycerol solution (50%)

specific activity

≥35 units/mg protein

mol wt

310-350 kDa

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

cow ... GLUD1(281785)

Application

L-glutamic dehydrogenase was used to catalyze the conversion of isocitrate into α-ketoglutarate and carbon dioxide.

Biochem/physiol Actions

Mammalian forms of this enzyme, including this bovine form, can use either NADP(H) or NAD(H) as coenzymes. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway by which ammonia can become bound to the α-carbon atom of an α-carboxylic acid and thus, is the only source of de novo amino acid synthesis in mammalian species.

The bovine enzyme is characterized by three sets of properties:
  • It has a reversible concentration-dependent association, producing higher molecular weight forms.
  • Forms tight enzyme-reduced coenzyme-substrate ternary complexes whose rates of dissociation modulate the steady-state reaction rates.
  • Exhibits a wide variety of effects from the binding of any of a number of nucleotide modifiers.

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

Physical form

50% glycerol solution

Analysis Note

Protein determined by biuret.

Other Notes

One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.3 at 25 °C, in the presence of ammonium ions.

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This Item
G2501G788210197734001
description

Type II, 50% glycerol solution, ≥35 units/mg protein

description

Type I, ammonium sulfate suspension, ≥40 units/mg protein

description

Type III, lyophilized powder, ≥20 units/mg protein

description

from beef liver

Gene Information

cow ... GLUD1(281785)

Gene Information

cow ... GLUD1(281785)

Gene Information

cow ... GLUD1(281785)

Gene Information

bovine ... GLUD1(281785)

specific activity

≥35 units/mg protein

specific activity

≥40 units/mg protein

specific activity

≥20 units/mg protein

specific activity

10 U/mg, ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)

biological source

bovine liver

biological source

-

biological source

bovine liver

biological source

bovine liver

form

glycerol solution (50%)

form

ammonium sulfate suspension

form

lyophilized powder

form

lyophilized

UniProt accession no.

P00366

UniProt accession no.

P00366

UniProt accession no.

P00366

UniProt accession no.

P00366

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

wet ice


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Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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