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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Available to ship TODAYfromMILWAUKEE | $78.20 |
About This Item
$78.20
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Available to ship TODAYDetails
usage
sufficient for 10 purifications
Quality Segment
greener alternative product characteristics
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
storage temp.
15-25°C
General description
Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns.
The purified genomic DNA is ready for immediate use in downstream applications such as PCR, restriction digestion, cloning and Southern blots.
Application
- in isolation of arbuscular mycorrhizal (AM) fungal spores DNA
- in the purification of cetyl trimethyl ammonium bromide (CTAB)-extracted genomic DNA
- in genomic DNA isolation from leaves
- PCR
- restriction endonuclease digestion
- cloning
- Southern blots
Biochem/physiol Actions
Features and Benefits
- Starting material: Up to 100 mg of plant tissue
- Expected yield: Up to 20 μg
- Elution volume: 100 - 200 μl
- Time required: < 40 min
- RNase treatment required: No
Other Notes
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| description sufficient for 10 purifications | description sufficient for 70 purifications | description sufficient for 35 purifications | description sufficient for 350 purifications |
| usage sufficient for 10 purifications | usage sufficient for 70 purifications | usage sufficient for 35 purifications | usage sufficient for 350 purifications |
| greener alternative category | greener alternative category , Aligned | greener alternative category | greener alternative category - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability - |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
| greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention | greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention | greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention | greener alternative product characteristics - |
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Kit Components Also Available Separately
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
supp_hazards
Storage Class
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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