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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Available to ship TODAYfromMILWAUKEE | $81.00 |
About This Item
$81.00
Available to ship TODAYDetails
usage
sufficient for 10 purifications
Quality Segment
sustainability
Sustainability features
storage temp.
15-25°C
General description
Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns.
The purified genomic DNA is ready for immediate use in downstream applications such as PCR, restriction digestion, cloning and Southern blots.
Application
- in isolation of arbuscular mycorrhizal (AM) fungal spores DNA
- in the purification of cetyl trimethyl ammonium bromide (CTAB)-extracted genomic DNA
- in genomic DNA isolation from leaves
- PCR
- restriction endonuclease digestion
- cloning
- Southern blots
Biochem/physiol Actions
Features and Benefits
- Starting material: Up to 100 mg of plant tissue
- Expected yield: Up to 20 μg
- Elution volume: 100 - 200 μl
- Time required: < 40 min
- RNase treatment required: No
Other Notes
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| description sufficient for 10 purifications | description sufficient for 350 purifications | description sufficient for 70 purifications | description sufficient for 70 purifications |
| usage sufficient for 10 purifications | usage sufficient for 350 purifications | usage sufficient for 70 purifications | usage sufficient for 70 purifications |
| greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention | greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention | greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention | greener alternative product characteristics Designing Safer Chemicals |
| sustainability Sustainability features | sustainability Sustainability features | sustainability Sustainability features | sustainability Sustainability features |
| greener alternative category | greener alternative category | greener alternative category | greener alternative category |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
supp_hazards
Storage Class
8A - Combustible corrosive hazardous materials
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
WGK
WGK 3
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