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Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

Name: Glucose-6-phosphate Dehydrogenase from baker′s yeast (<I>S. cerevisiae</I>)
Brand: SIGMA
Price: 143 USD
Availability: InStock

Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

Synonym(s):

G-6-P-DH, Zwischenferment

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Size/SKU	Availability	Price
100 units	Check Cart for Availability	$143.00
250 units	Check Cart for Availability	$286.00
500 units	Check Cart for Availability	$493.00
1000 units	Check Cart for Availability	$768.00

About This Item

CAS Number:

9001-40-5

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-602-6

MDL number:

MFCD00081656

EC Number:

1.1.1.49 (BRENDA, IUBMB)

Specific activity:

200-400 units/mg protein (modified Warburg-Christian)

$143.00

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Properties

type

Type IX

Quality Segment

200

form

lyophilized powder

specific activity

200-400 units/mg protein (modified Warburg-Christian)

mol wt

128 kDa

β-NADP and β-NADPH content

≤10 mmol/mol

application(s)

agriculture

shipped in

dry ice

storage temp.

−20°C

Description

General description

Research area: Cell Signaling

Glucose-6-phosphate dehydrogenase (G6PD) is a key metabolic enzyme of the pentose phosphate pathway. In S. cerevisiae, it is encoded by the ZWF1 gene. G6PD exists as a tetramer in its active form.

Application

Glucose-6-phosphate dehydrogenase is used:

To test ketose reductase activity in developing maize endosperm.
For recycling microassay of β-NADP and β-NADPH.
To measure the intracellular levels of NADPH and total NADP.
To measure the nicotinamide adenine dinucleotide (NAD) kinase kinetic assay activity.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase catalyzes the rate-limiting step in the pentose phosphate pathway. Its function involves the conversion of glucose-6-phosphate to 6-phosphogluconolacetone while generating NADPH, which is essential for the regeneration of glutathione The glutathione system utilizes nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) to effectively eliminate excess hydrogen peroxide. Glucose-6-phosphate dehydrogenase (G6PD) plays an important role in regulating cell growth and survival. Their levels are higher in cells undergoing normal and neoplastic growth. Increased glucose-6-phosphate dehydrogenase activity plays a pivotal role in preventing reactive oxygen species mediated cell death. Glucose-6-phosphate dehydrogenase is over expressed in several cancers whereas its activity is reduced in hyperglycemia. A deficiency in glucose-6-phosphate dehydrogenase causes hemolysis.

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Physical form

Lyophilized powder essentially sulfate-free, containing approx. 20% sodium citrate

Other Notes

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

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This Item	G6378	G7877	H8629
Sigma-Aldrich G4134 Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)	Sigma-Aldrich G6378 Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)	Sigma-Aldrich G7877 Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)	Sigma-Aldrich H8629 Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)
description Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)	description Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)	description Type VII, ammonium sulfate suspension, ≥200 units/mg protein	description lyophilized powder
specific activity 200-400 units/mg protein (modified Warburg-Christian)	specific activity 200-400 units/mg protein (modified Warburg-Christian)	specific activity ≥200 units/mg protein	specific activity -
form lyophilized powder	form lyophilized powder	form ammonium sulfate suspension	form lyophilized powder
application(s) agriculture	application(s) diagnostic assay manufacturing	application(s) diagnostic assay manufacturing	application(s) -
mol wt 128 kDa	mol wt 128 kDa	mol wt 128 kDa	mol wt -
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
shipped in dry ice	shipped in dry ice	shipped in -	shipped in -

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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