Skip to Content
MilliporeSigma

Skip To

G4511

β-Glucosidase from almonds

lyophilized powder, 10-50 units/mg solid

greener alternative

Greener Alternative

Synonym(s):

β-D-Glucoside glucohydrolase

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
100 units
Check Cart for Availability
$217.00
250 units

Available to ship TODAYfromMILWAUKEE

$432.00
1000 units

Available to ship TODAYfromMILWAUKEE

$1,450.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-589-7
MDL number:
EC Number:
Specific activity:
10-50 units/mg solid

$217.00


Check Cart for Availability

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


form

lyophilized powder

Quality Segment

specific activity

10-50 units/mg solid

mol wt

50-75 kDa

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

impurities

salt, essentially free

greener alternative category

storage temp.

2-8°C

General description

β-Glucosidase from almonds belongs to the family 1 of the glycoside hydrolases. Almonds contain prunasin hydrolases (PH) and amygdalin hydrolase. Nine types of PHs are associated with almonds. PHs have signal peptide sequence and correspond to molecular weight in the range 50 to 75 kDa. PHs also have ITENG, NEP and INKKGIEYY motifs conserved and have N-glycosylation sites.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.

Application

β-glucosidase is also used in the synthesis of glucosides and fucosides with various potential applications in pharmaceutical, cosmetic and detergent industries, hydrolytic removal of aglycone moiety from flavonoid and isoflavonoid glycosides, flavor enhancement of fruit juices and wine, and biosynthesis of oligosaccharides.
β-Glucosidase from almonds has been used:
  • in enzyme inhibition studies by 1,5-dideoxy-1,5-imino-d-xylitol(DIX) derivates
  • as a control in for comparison of soybean isofavone glycosides degradation by β-Glucosidase from Talaromyce leycettanus
  • as a medium component during saccharification and fermentation of yeast

Biochem/physiol Actions

Prunasin hydrolase (PH) is a β-glucosidase that degrades prunasin to mandelonitrile and glucose. Amygdalin hydrolase hydrolyzes amygdalin to prunasin and glucose. β-Glucosidase is mesostable and is sensitive to temperature at 60 °C and above. It is highly sensitive to high pressure.
βglucosidase is involved in the hydrolysis of β-glycosidic bonds connecting carbohydrate residues in β-D-glycosides. They convert cellobiose and cellooligosaccharides produced by the endo and exoglucanases to glucose.

Preparation Note

Chromatographically purified

Other Notes

One unit will liberate 1.0 μmole of glucose from salicin per min at pH 5.0 at 37 °C.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
G0535G365110065
description

lyophilized powder, 10-50 units/mg solid

description

buffered aqueous glycerol solution, ≥0.05 unit/mL

description

lyophilized powder, ≥50 units/mg protein

description

powder, ~30 U/mg

specific activity

10-50 units/mg solid

specific activity

-

specific activity

≥50 units/mg protein

specific activity

~30 U/mg

form

lyophilized powder

form

buffered aqueous glycerol solution

form

lyophilized powder

form

powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

50-75 kDa

mol wt

-

mol wt

-

mol wt

Mr ~51000

impurities

salt, essentially free

impurities

-

impurities

-

impurities

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100


Still not finding the right product?

Explore all of our products under β-Glucosidase from almonds


pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library



Questions

1–2 of 2 Questions  
  1. Cual es el volumen final de cada envase una vez reconstituido ?

    1 answer
    1. The solubility of this product is tested at 2 mg/mL. For assay purposes, the material is reconstituted at 1.2 – 2.4 U/mL. The final volume is user-defined, not preassigned. The enzyme is typically reconstituted to the desired activity (U/mL).

      Helpful?

  2. What is the recommended method for storing stock solutions of item G4511, β-Glucosidase from almonds?

    1 answer
    1. The β-Glucosidase from almonds should be stored in a solvent at -80°C for up to 6 months, or at -20°C for up to 1 month.

      Helpful?

Reviews

No rating value

Active Filters