The solubility of this product is tested at 2 mg/mL. For assay purposes, the material is reconstituted at 1.2 – 2.4 U/mL. The final volume is user-defined, not preassigned. The enzyme is typically reconstituted to the desired activity (U/mL).
G4511
β-Glucosidase from almonds
lyophilized powder, 10-50 units/mg solid
Sustainability features
Synonym(s):
β-D-Glucoside glucohydrolase
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|---|---|
100 units | Available to ship TODAYfromMILWAUKEE | $225.00 |
250 units | Available to ship TODAYfromMILWAUKEE | $448.00 |
1000 units | Available to ship TODAYfromMILWAUKEE | $1,500.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-589-7
MDL number:
EC Number:
Specific activity:
10-50 units/mg solid
$225.00
Available to ship TODAYDetails
form
lyophilized powder
Quality Segment
specific activity
10-50 units/mg solid
mol wt
50-75 kDa
sustainability
Sustainability features
impurities
salt, essentially free
storage temp.
2-8°C
General description
β-Glucosidase from almonds belongs to the family 1 of the glycoside hydrolases. Almonds contain prunasin hydrolases (PH) and amygdalin hydrolase. Nine types of PHs are associated with almonds. PHs have signal peptide sequence and correspond to molecular weight in the range 50 to 75 kDa. PHs also have ITENG, NEP and INKKGIEYY motifs conserved and have N-glycosylation sites.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.
Application
β-glucosidase is also used in the synthesis of glucosides and fucosides with various potential applications in pharmaceutical, cosmetic and detergent industries, hydrolytic removal of aglycone moiety from flavonoid and isoflavonoid glycosides, flavor enhancement of fruit juices and wine, and biosynthesis of oligosaccharides.
β-Glucosidase from almonds has been used:
- in enzyme inhibition studies by 1,5-dideoxy-1,5-imino-d-xylitol(DIX) derivates
- as a control in for comparison of soybean isofavone glycosides degradation by β-Glucosidase from Talaromyce leycettanus
- as a medium component during saccharification and fermentation of yeast
Biochem/physiol Actions
Prunasin hydrolase (PH) is a β-glucosidase that degrades prunasin to mandelonitrile and glucose. Amygdalin hydrolase hydrolyzes amygdalin to prunasin and glucose. β-Glucosidase is mesostable and is sensitive to temperature at 60 °C and above. It is highly sensitive to high pressure.
βglucosidase is involved in the hydrolysis of β-glycosidic bonds connecting carbohydrate residues in β-D-glycosides. They convert cellobiose and cellooligosaccharides produced by the endo and exoglucanases to glucose.
Preparation Note
Chromatographically purified
Other Notes
One unit will liberate 1.0 μmole of glucose from salicin per min at pH 5.0 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| description lyophilized powder, 10-50 units/mg solid | description lyophilized powder, ≥2 units/mg solid | description lyophilized, powder, ≥4 U/mg | description Type V, ammonium sulfate suspension, 40-80 units/mg protein |
| specific activity 10-50 units/mg solid | specific activity ≥2 units/mg solid | specific activity ≥4 U/mg | specific activity 40-80 units/mg protein |
| form lyophilized powder | form lyophilized powder | form powder | form ammonium sulfate suspension |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| mol wt 50-75 kDa | mol wt 135 kDa | mol wt Mr ~135000 | mol wt - |
| impurities salt, essentially free | impurities salt, essentially free | impurities - | impurities - |
| Quality Level 200 | Quality Level 300 | Quality Level 100 | Quality Level 200 |
Still not finding the right product?
Explore all of our products under β-Glucosidase from almonds
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
Cual es el volumen final de cada envase una vez reconstituido ?
1 answer-
Helpful?
-
-
What is the recommended method for storing stock solutions of item G4511, β-Glucosidase from almonds?
1 answer-
The β-Glucosidase from almonds should be stored in a solvent at -80°C for up to 6 months, or at -20°C for up to 1 month.
Helpful?
-