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G5166

PNGase F Glycosidase

Removes N-linked glycans, suitable for mass spectrometry, from Elizabethkingia miricola

Synonym(s):

N-Glycosidase F, Peptide N-glycosidase

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Pack SizeSKUAvailabilityPrice
50 units
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$367.00
100 units
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$638.00

About This Item

CAS Number:
83534-39-8
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
3.5.1.52 (BRENDA, IUBMB)
MDL number:
MFCD06656235
Specific activity:
≥20000 units/mg protein
Biological source:
bacterial (Elizabethkingia miricola)

$367.00

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Product Name

PNGase F from Elizabethkingia miricola, buffered aqueous solution

biological source

bacterial (Elizabethkingia miricola)

Quality Level

200

conjugate

(N-linked)

form

buffered aqueous solution

specific activity

≥20000 units/mg protein

mol wt

36 kDa

shipped in

wet ice

storage temp.

2-8°C

General description

PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides.

Application

Used to deglycosylate protein.
PNGase F from Elizabethkingia miricola has been used for the deglycosylation of the proteins.[1]

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Physical form

Supplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA

Other Notes

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

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PNGase F from Elizabethkingia miricola buffered aqueous solution

Sigma-Aldrich

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Sigma-Aldrich

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Sigma-Aldrich

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Sigma-Aldrich

F8435

PNGase F from Elizabethkingia meningoseptica

specific activity

≥20000 units/mg protein

specific activity

-

specific activity

≥1000 U/mg

specific activity

≥1,000 U/mg

biological source

bacterial (Elizabethkingia miricola)

biological source

bacterial (Elizabethkingia meningoseptica)

biological source

-

biological source

-

form

buffered aqueous solution

form

powder

form

ready-to-use solution

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

36 kDa

mol wt

~36 kDa

mol wt

~36 kDa

mol wt

~36 kDa

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000529520
0000529520
0000496109
0000496109
0000379195

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Related Content

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

Glycobiology Brochure

Glycobiology and Glycoproteomics Brochure

Data Sheet - G5166

Instructions

Ying-Nai Wang et al.
STAR protocols, 3(1), 101198-101198 (2022-03-05)
Immunotherapy via PD-1/PD-L1 blockade is a promising strategy to eradicate cancer cells. However, the PD-L1 pathological level is inconsistent with the therapeutic response and is not a reliable biomarker to stratify patients for anti-PD-1/PD-L1 therapy. Here, we describe patient sample deglycosylation
A novel humanized GLP-1 receptor model enables both affinity purification and Cre-LoxP deletion of the receptor
Jun LS, et al.
PLoS ONE, 9(4), e93746-e93746 (2014)
Identification and Characterization of a Novel Prokaryotic Peptide N-GLYCOSIDASE FROM ELIZABETHKINGIA MENINGOSEPTICA
Sun G, et al.
The Journal of Biological Chemistry, 290(12), 7452-7462 (2015)
View All Related Papers

Global Trade Item Number

SKUGTIN
G5166-100UN04061833633441
G5166-50UN04061832856568

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