Unfortunately, Product G6539 is not expected to work for turboGFP.
G6539
Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse
clone GFP-20, ascites fluid
Synonym(s):
GFP Antibody - Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse, Gfp Antibody Sigma, Gfp Monoclonal Antibody
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| Size/SKU | Availability | Price |
|---|---|---|
25 μL | Estimated to ship onAugust 17, 2026fromMILWAUKEE | $185.00 |
100 μL | Estimated to ship onAugust 17, 2026fromMILWAUKEE | $581.00 |
0.2 mL | Estimated to ship onAugust 17, 2026fromMILWAUKEE | $727.00 |
About This Item
Conjugate:
unconjugated
Clone:
GFP-20, monoclonal
Application:
DB, ELISA (i), WB
Citations:
170
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
GFP-20, monoclonal
mol wt
antigen 27 kDa
contains
15 mM sodium azide
packaging
antibody small pack of 25 μL
technique(s)
dot blot: suitable, indirect ELISA: suitable, western blot: 1:2,000 using purified recombinant GFP preparation
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Monoclonal Anti-Green Fluorescent Protein (GFP) (mouse IgG1 isotype) is derived from the GFP-20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a GFP tagged fusion protein. GFP is a 27 kDa (238 amino acid) protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca2+-activated photoprotein aequorin to GFP. GFP is a unique tool to monitor dynamic processes in a variety of living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. Exogenous substrates and cofactors are not required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP signals can be quantified by flow cytometry, confocal scanning laser microscopy, and fluorometric assays. Indeed, many recombinant proteins have been engineered with GFP tags to facilitate the detection, isolation and purification of the proteins.
The Green Fluorescent Protein (GFP) is derived from the jellyfish Aequorea Victoria and is often used as a gene expression marker. Thus, antibodies to GFP can facilitate the detection and analysis of GFP labeled nucleic acids and proteins. Monoclonal Anti-Green Fluorescent Protein (GFP) antibody is specific for GFP and GFP-labeled biomolecules.
Immunogen
GFP tagged fusion protein
GFP tagged fusion protein.
Application
Monoclonal Anti-Green Fluorescent Protein (GFP) recognizes GFP (27 kDa) using immunoblotting, dot blot, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation and enzyme linked immunosorbent assay (ELISA). The antibody reacts with fusion proteins expressed by prokaryotes expression vectors.
Monoclonal Anti-Green Fluorescent Protein (GFP) antibody is suitable for use in western blot and ChIP assays.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description clone GFP-20, ascites fluid | description clone GSN24, purified from hybridoma cell culture | description clone G-A-5, purified from hybridoma cell culture | description clone G-A-5, ascites fluid |
| clone GFP-20, monoclonal | clone GSN24, monoclonal | clone G-A-5, monoclonal | clone G-A-5, monoclonal |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| antibody form ascites fluid | antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture, purified immunoglobulin | antibody form ascites fluid |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| technique(s) dot blot: suitable, western blot: 1:2,000 using purified recombinant GFP preparation, indirect ELISA: suitable | technique(s) western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells | technique(s) immunocytochemistry: suitable, immunohistochemistry: suitable, indirect immunofluorescence: 2.5-5 μg/mL using alcohol-fixed sections of rat brain/cerebellum., western blot: suitable | technique(s) immunoblotting: suitable, immunocytochemistry: suitable, immunohistochemistry: 1:400 using rat brain sections (alcohol-fixed), indirect immunofluorescence: suitable, microarray: suitable |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Hi, I was wondering if this antibody is suitable for turboGFP. Could you expand on this? Thanks
1 answer-
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