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Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal

Name: Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal
Brand: SIGMA
Price: 551 USD
Availability: InStock

clone GSN24, purified from hybridoma cell culture

Synonym(s):

Anti-GFP

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Size/SKU	Availability	Price
200 μg	Check Cart for Availability	$551.00

About This Item

NACRES:

NA.56

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

GSN24, monoclonal

Application:

Citations:

$551.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GSN24, monoclonal

form

buffered aqueous solution

mol wt

antigen 27 kDa

concentration

~2 mg/mL

technique(s)

western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells

isotype

IgG1

shipped in

dry ice

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma GSN24 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice.

Application

Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal has been used in: western blotting, enzyme linked immunosorbent assay (ELISA), immunofluorescence labeling.

Biochem/physiol Actions

Green Fluorescent Protein (GFP) is a unique tool in cellular and molecular biology research to monitor gene expression and protein localization in living cells. In the jellyfish, A. victoria, GFP transduces the excitation energy resulting from emission of blue light of the photoprotein aequorin and reemits it as green light.

The antibody reacts specifically with GFP fusion proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2–8 °C for up to one month. For extended storage, store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	G1546	SAB4200681	G6539
Sigma-Aldrich G6795 Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal	Sigma-Aldrich G1546 Anti-Green Fluorescent Protein (GFP) antibody, Mouse monoclonal	Sigma-Aldrich SAB4200681 Anti-Green Fluorescent Protein (GFP) antibody, Mouse monoclonal	Sigma-Aldrich G6539 Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse
description clone GSN24, purified from hybridoma cell culture	description clone GSN149, purified from hybridoma cell culture	description clone GFP-20, purified from hybridoma cell culture	description clone GFP-20, ascites fluid
conjugate unconjugated	conjugate unconjugated	conjugate -	conjugate unconjugated
clone GSN24, monoclonal	clone GSN149, monoclonal	clone GFP-20, monoclonal	clone GFP-20, monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source mouse
antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form purified antibody	antibody form ascites fluid
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
mol wt antigen 27 kDa	mol wt -	mol wt antigen 27 kDa	mol wt antigen 27 kDa

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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