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Anti-Mouse IgG (whole molecule)–Gold antibody produced in goat

Name: Anti-Mouse IgG (whole molecule)–Gold antibody produced in goat
Brand: SIGMA

affinity isolated antibody, aqueous glycerol suspension, 10 nm (colloidal gold)

Synonym(s):

Goat anti-mouse IgG

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162643

Conjugate:

gold conjugate

Clone:

polyclonal

Application:

—

Citations:

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Properties

biological source

goat

Quality Segment

200

conjugate

gold conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

aqueous glycerol suspension

particle size

10 nm (colloidal gold)

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Binding activity is determined using a modification of the dot blot assay of Brada and Roth. In this assay a 1 mg/mL solution of mouse IgG is serially diluted in phosphate buffered saline, 1 mL of each dilution is then applied to nitrocellulose paper. Binding activity is defined as the minimum amount of mouse IgG detectable as a visible pink-red spot after a 1 hour incubation with the gold conjugate diluted to an A520 of0.25. Relative order of singlets, doublets, triplets or greater: Singlets>Doublets>Triplets. Clustering data determined by floating the electron microscopy (EM) nickel grid on conjugate diluted 1:100 in Tris buffer, pH 8.0, containing 1% BSA, for 30-60 minutes. The grid is evaluated by EM after drying. One hundred particles are scanned to determine clustering.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (whole molecule)-Gold antibody is specific for mouse IgG.

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Immunogold labeling is a technique whereing antibodies, especially secondary antibodies, are labeled with colloidal gold. These gold conjugated antibodies generate electron dense particles that are visable using electron microscopy.

Immunogen

Mouse IgG purified from normal mouse serum

Application

Anti-Mouse IgG (whole molecule)-Gold antibody is suitable for use in inductively coupled plasma mass spectrometry (ICPMS)-Linked DNA Assay ((1:100). The product can also be used for immunoelectron microscopy (1:20).

Goat polyclonal anti-mouse IgG (whole molecule)–Gold antibody may be used as a primary or secondary antibody that enables the labeling of mouse IgG for detection by electron microscopy.

Physical form

Colloidal suspension in Tris buffered saline, pH 8.2, with 30% glycerol (v/v), 1% bovine serum albumin (w/v), and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	G7527	G7777	G7277
Sigma-Aldrich G7652 Anti-Mouse IgG (whole molecule)–Gold antibody produced in goat	Sigma-Aldrich G7527 Anti-Mouse IgG (whole molecule)–Gold antibody produced in goat	Sigma-Aldrich G7777 Anti-Mouse IgG (whole molecule)–Gold antibody produced in goat	Sigma-Aldrich G7277 Anti-Rabbit IgG (whole molecule)−Gold antibody produced in goat
description affinity isolated antibody, aqueous glycerol suspension, 10 nm (colloidal gold)	description affinity isolated antibody, aqueous glycerol suspension, 5 nm (colloidal gold)	description affinity isolated antibody, aqueous glycerol suspension, 10 nm (colloidal gold)	description affinity isolated antibody, aqueous glycerol suspension, 5 nm (colloidal gold)
biological source goat	biological source goat	biological source goat	biological source goat
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate gold conjugate	conjugate gold conjugate	conjugate gold conjugate	conjugate gold conjugate
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
form aqueous glycerol suspension	form aqueous glycerol suspension	form aqueous glycerol suspension	form aqueous glycerol suspension
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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