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Monoclonal Anti-HLA Class I Antigen antibody produced in mouse

Name: Monoclonal Anti-HLA Class I Antigen antibody produced in mouse
Brand: SIGMA
Price: 279 USD
Availability: InStock

clone W6/32, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-HLA Class I Antigen

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50 test	Check Cart for Availability	$279.00
100 test	Check Cart for Availability	$501.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

W6/32, monoclonal

Application:

FACS, IF, IP

Citations:

$279.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

W6/32, monoclonal

form

buffered aqueous solution

species reactivity

monkey, human

technique(s)

flow cytometry: 5 μL using 1 × 10⁶ cells, immunoprecipitation (IP): suitable, indirect immunofluorescence: 1:10 using acetone-fixed human tonsil frozen sections.

isotype

IgG2a

UniProt accession no.

P04439

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... HLA-A(3105), HLA-B(3106), HLA-C(3107)

Description

General description

Class I proteins are highly polymorphic, in which HLA-B seems to be the most polymorphic gene in the human genome. HLA-A, -B, -C are classical class I proteins and are heterodimers. HLA-A, -B, -C consists of a polymorphic transmembrane α heavy chain and an invariant water-soluble light chain. HLA-B is located on human chromosome 6p21.

The previously assigned protein identifier P01891 has been merged into P04439. Full details can be found on the UniProt database.

Immunogen

human tonsil leukocyte membrane preparation.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

HLA class I molecules like HLA-A, -B and −C help in the presentation of endogenous antigen to CD8+ (cluster of differentiation 8) T cells.

Recognizes the human HLA Class I antigen expressed on most human nucleated cells. The antibody reacts with a monomorphic epitope on the 44-45 kDa α-chain polypeptide of human class I HLA molecules (HLA-A,B,C). The antibody may be used in immunoprecipitation, complement mediated cytotoxicity, flow cytometry and immunohistochemical staining of acetone-fixed, frozen sections and cell smears. It cross-reacts with cells from African and Asian apes and old world monkeys. No reactivity is seen with cells from most new world monkeys or from non-primates. The epitope recognized by this antibody is sensitive to routine formalin fixation and paraffin embedding.
4th Workshop: code no. P1
5th Workshop: code nos. BP166, BP288, BP407, B 005

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	F5662	SAB4700640	SAB4700638
Sigma-Aldrich H1650 Monoclonal Anti-HLA Class I Antigen antibody produced in mouse	Sigma-Aldrich F5662 Monoclonal Anti-HLA Class I Antigen−FITC antibody produced in mouse	Sigma-Aldrich SAB4700640 Monoclonal Anti-HLA Class I-PE antibody produced in mouse	Sigma-Aldrich SAB4700638 Monoclonal Anti-HLA Class I-Biotin antibody produced in mouse
description clone W6/32, purified immunoglobulin, buffered aqueous solution	description clone W6/32, purified immunoglobulin, buffered aqueous solution	description clone W6/32, purified immunoglobulin, buffered aqueous solution	description clone W6/32, purified immunoglobulin, buffered aqueous solution
Gene Information human ... HLA-A(3105), HLA-B(3106), HLA-C(3107)	Gene Information human ... HLA-A(3105), HLA-B(3106), HLA-C(3107)	Gene Information human ... HLA-A(3105), HLA-B(3106), HLA-C(3107)	Gene Information human ... HLA-A(3105), HLA-B(3106), HLA-C(3107)
biological source mouse	biological source mouse	biological source mouse	biological source mouse
species reactivity monkey, human	species reactivity monkey, human	species reactivity bovine, human, nonhuman primates, feline	species reactivity human
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin
conjugate unconjugated	conjugate FITC conjugate	conjugate phycoerythrin (R-PE) conjugate	conjugate biotin conjugate
clone W6/32, monoclonal	clone W6/32, monoclonal	clone W6/32, monoclonal	clone W6/32, monoclonal

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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