This product is a solution containing 1 nanomole or 1000 picomoles of Cytochrome P450 3A4. The concentration of several parameters varies from lot to lot and is reported in the product Certificate of Analysis. As noted in the 'PROPERTIES' section, each vial will have a range of 3.5-25 mg protein/mL, 50-150 nmol/mL P450, and 6-14 nmol/mg protein. For example, a recent lot was found to have a P450 concentration of 13 nmol/mg protein and 5.1 mg protein/mL. The volume of the 1000 picomole package for this lot is 1.5 uL. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/h5042#product-documentation
H5042
Cytochrome P450 3A4 human
recombinant, expressed in E. coli
Synonym(s):
Cytochrome P450, purified, soluble, 1,4-cineole 2-exo-monooxygenase, Albendazole sulfoxidase, CYP3A3, CYP3A4, Cytochrome P450 NF-25, Cytochrome P450-PCN1
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| Size/SKU | Availability | Price |
|---|---|---|
1000 pmol | Estimated to ship onOctober 21, 2027fromSAINT LOUIS | $746.00 |
About This Item
Form:
liquid
Assay:
3.5—25 mg protein/mL (Bradford)
Biological source:
human
Recombinant:
expressed in E. coli
Mol wt:
57318.7 g/mol
biological source
human
Quality Segment
recombinant
expressed in E. coli
description
purified, soluble
assay
3.5—25 mg protein/mL (Bradford)
form
liquid
specific activity
12-200 units/mg protein
mol wt
57318.7 g/mol
concentration
3.5-25 mg protein/mL (protein), 50-150 nmol/mL (P450), 6-14 nmol/mg protein (specific content)
technique(s)
cell based assay: suitable
solubility
water: soluble
NCBI accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CYP3A4(1576)
General description
Research area: IMMUNO AND CKS
Cytochrome P450 3A4 (CYP3A4) indicates a mammalian enzyme (CYP) family 3, subfamily A, gene 4. There are 57 CYP genes and 33 pseudogenes in humans, organized into 18 families and 42 subfamilies. Among these, the 3A4 isoform (CYP3A4) is the most prevalent and is primarily found in the liver and gastrointestinal tract.
Cytochrome P450 3A4 (CYP3A4) indicates a mammalian enzyme (CYP) family 3, subfamily A, gene 4. There are 57 CYP genes and 33 pseudogenes in humans, organized into 18 families and 42 subfamilies. Among these, the 3A4 isoform (CYP3A4) is the most prevalent and is primarily found in the liver and gastrointestinal tract.
Application
Cytochrome P450 3A4 human has been used in circular dichroism analysis.
Biochem/physiol Actions
Cytochrome P450 3A4 (CYP3A4) is one of the isoforms of cytochrome P450 (P450s). Cytochrome P450s (P450s) plays a vital role in processing endogenous compounds and xenobiotics, including pollutants, environmental compounds, and drug molecules. CYP3A4 oxidizes more than 90% of marketed drugs through various metabolic processes.CYP3A4 mRNA expression is upregulated in pediatric Crohn’s disease. Moreover, it provides high tolerance against herbicides such as mefenacet, amiprofos-methyl, pyributicarb, norflurazon, trifluralin, pendimethalin, chlorotoluron, and chloroacetamides.
Other Notes
One unit will convert 1.0 nanomole of testosterone to 6-beta-testosterone per minute at pH 7.4 at 37 °C.
Supplied as purified soluble proteins, rather than microsomal preparations.
1 of 1
This Item | |||
|---|---|---|---|
| description recombinant, expressed in E. coli | description 3A4 isozyme microsomes, with P450 reductase and cytochrome b5, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4) | description - | description 1A2 Isozyme Microsomes, with P450 Reductase and cytochrome b5, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4) |
| assay 3.5—25 mg protein/mL (Bradford) | assay - | assay - | assay - |
| technique(s) cell based assay: suitable | technique(s) co-immunoprecipitation (co-IP): suitable | technique(s) - | technique(s) - |
| recombinant expressed in E. coli | recombinant expressed in baculovirus infected insect cells (BTI-TN-5B1-4) | recombinant expressed in Pichia pastoris | recombinant expressed in baculovirus infected insect cells (BTI-TN-5B1-4) |
| biological source human | biological source human | biological source human | biological source human |
| concentration 6-14 nmol/mg protein (specific content), 50-150 nmol/mL (P450), 3.5-25 mg protein/mL (protein) | concentration ≥2 mg/mL (Bichinchonic acid method) | concentration - | concentration - |
| form liquid | form solution | form dry powder | form solution |
Storage Class
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Hello, Could you please provide more details about this product? Specifically, I would like to know the volume of the aliquot. Also, could you provide a more precise concentration in mg/mL?
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