This product is tested for the expansion of TF-1 cells. The activity is reported as the ED50 in nanograms per milliliter. The activity in International Units has not been established. The ED50 value is reported on the lot-specific Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/h5166#product-documentation
H5166
Erythropoietin human
EPO, recombinant, expressed in HEK 293 cells, suitable for cell culture
Synonym(s):
EPO
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About This Item
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
dimer 36 kDa (glycosylated)
Impurities:
≤1 EU/μg
Pricing and availability is not currently available.
biological source
human
Quality Segment
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤5.0 ng/mL ED50
quality
endotoxin tested
mol wt
dimer 36 kDa (glycosylated)
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... EPO(2056)
General description
Recombinant human erythropoietin (EPO) is expressed in HEK 293 cells and supplied as a glycosylated lyophilized protein suitable for mammalian cell culture research. The mature protein contains three N‑linked glycosylation sites, which are important for its structural integrity and stability in vitro.
Application
Recombinant human EPO has been used in mammalian cell culture research applications, including studies of erythroid progenitor cell growth, differentiation, and in vitro cell‑based bioassays.
Biochem/physiol Actions
Erythropoietin is a glycoprotein that is the principal regulator of red blood cell growth and differentiation.
Erythropoietin (EPO) is a glycoprotein growth factor that supports proliferation and differentiation of erythroid progenitor cells through interaction with the erythropoietin receptor. Erythropoietin stimulates erythropoiesis by inducing growth and differentiation of burst forming units and colony forming units into mature red blood cells.
Preparation Note
Human EPO is expressed as a glycosylated 36 kDa monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Analysis Note
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
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This Item | |||
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| description EPO, recombinant, expressed in HEK 293 cells, suitable for cell culture | description recombinant, expressed in NSO cells, ≥90% (SDS-PAGE), lyophilized powder, suitable for cell culture | description >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder | description recombinant, expressed in HEK 293 cells, suitable for cell culture |
| recombinant expressed in HEK 293 cells | recombinant expressed in NSO cells | recombinant expressed in NSO cells | recombinant expressed in HEK 293 cells |
| assay ≥95% (SDS-PAGE) | assay ≥90% (SDS-PAGE) | assay >90% (SDS-PAGE) | assay ≥98% (SDS-PAGE) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| biological source human | biological source mouse | biological source human | biological source - |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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