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H6147

HEPES

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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25 g

Available to ship TODAYfromMILWAUKEE

$99.40
100 g

Available to ship TODAYfromMILWAUKEE

$307.00
500 g

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$1,070.00
$909.50

About This Item

Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
UNSPSC Code:
12161704
NACRES:
NA.75
PubChem Substance ID:
EC Number:
230-907-9
Beilstein/REAXYS Number:
883043
MDL number:

$99.40


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product line

BioXtra

Quality Segment

assay

≥99.5% (titration)

form

powder

technique(s)

cell culture | embryo: suitable

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 500 mg/mL, clear, colorless

absorption

≤0.05 at 290 in H2O at 33%

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

General description

Useful pH range: 6.8 - 8.2
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.

Application

HEPES has been used:
  • As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells
  • As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production
  • in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.
This product has been qualified for use in cell and mouse embryo culture.

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H4034H7523391338
description

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

description

BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

description

BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

description

ULTROL® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer

assay

≥99.5% (titration)

assay

≥99.5% (titration)

assay

≥99.5% (titration)

assay

≥99% dry basis (titration)

technique(s)

cell culture | embryo: suitable

technique(s)

cell culture | mammalian: suitable, competitive inhibition ELISA: suitable

technique(s)

-

technique(s)

-

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

100

product line

BioXtra

product line

-

product line

BioXtra

product line

-

form

powder

form

crystalline powder

form

powder

form

crystalline powder

solubility

water: 500 mg/mL, clear, colorless

solubility

-

solubility

H2O: 1 M, clear, colorless

solubility

water: 1 M


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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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