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H8509

β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides

Type V, lyophilized powder, 250-750 units/mg protein

Synonym(s):

(R)-3-Hydroxybutanoate:NAD+ oxidoreductase, 3-HBDH

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Size/SKUAvailabilityPrice
25 units
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$1,620.00

About This Item

CAS Number:
9028-38-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-830-6
MDL number:
MFCD00130633
EC Number:
1.1.1.30 (BRENDA, IUBMB)
Specific activity:
250-750 units/mg protein

$1,620.00

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type

Type V

Quality Segment

form

lyophilized powder

specific activity

250-750 units/mg protein

purified by

chromatography

foreign activity

lactate dehydrogenase ≤0.05%, malate dehydrogenase ≤0.1%

storage temp.

−20°C

Application

Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).

Biochem/physiol Actions

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

Physical form

Lyophilized powder containing Tris buffer salts

Other Notes

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

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β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides Type V, lyophilized powder, 250-750 units/mg protein

Sigma-Aldrich

H8509

β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei lyophilized powder, ≥200 units/mg protein

Sigma-Aldrich

H9408

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei

Bacteriochlorophyll from Rhodopseudomonas sphaeroides

Sigma-Aldrich

B5906

Bacteriochlorophyll from Rhodopseudomonas sphaeroides

Aldehyde Dehydrogenase, Yeast Aldehyde Dehydrogenase, Yeast, CAS 9028-88-0, is a native aldehyde dehydrogenase from yeast that catalyzes the oxidation of acetaldehyde to acetic acid.

Sigma-Aldrich

126925

Aldehyde Dehydrogenase, Yeast

description

Type V, lyophilized powder, 250-750 units/mg protein

description

lyophilized powder, ≥200 units/mg protein

description

-

description

Aldehyde Dehydrogenase, Yeast, CAS 9028-88-0, is a native aldehyde dehydrogenase from yeast that catalyzes the oxidation of acetaldehyde to acetic acid.

specific activity

250-750 units/mg protein

specific activity

≥200 units/mg protein

specific activity

-

specific activity

≥19 units/mg protein

form

lyophilized powder

form

lyophilized powder

form

powder

form

lyophilized solid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

foreign activity

malate dehydrogenase ≤0.1%, lactate dehydrogenase ≤0.05%

foreign activity

malic dehydrogenase ≤0.1%, lactic dehydrogenase ≤0.05%

foreign activity

-

foreign activity

alcohol dehydrogenase ≤0.01 U/mg, oxidase ≤0.01 U/mg, NADH oxidase ≤0.01 U/mg, lactate dehydrogenase ≤0.01 U/mg

purified by

chromatography

purified by

chromatography

purified by

-

purified by

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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