Stock solutions prepared in water or 10 mM potassium phosphate buffer pH 8.0 lose approximately 1% activity per month when stored at 4°C.
H8629
Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)
lyophilized powder
Synonym(s):
HK/G6PD
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| Size/SKU | Availability | Price |
|---|---|---|
500 units | Available to ship TODAYfromMILWAUKEE | $146.00 |
5000 units | Available to ship TODAYfromMILWAUKEE | $1,140.00 |
About This Item
form
lyophilized powder
Quality Segment
glucose-6-phosphate dehydrogenase activity
≥75 units/mg protein (biuret)
hexokinase activity
≥65 units/mg protein
purified by
crystallization
storage temp.
−20°C
General description
Research area: Cell signaling
Hexokinase and Glucose-6-phosphate Dehydrogenase are intracellular enzymes found distributed widely in the living cells.
Hexokinase and Glucose-6-phosphate Dehydrogenase are intracellular enzymes found distributed widely in the living cells.
Enzyme Commission numbers 2.7.1.1 + 1.1.1.49
Application
Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae) has been used to evaluate the adenylate kinase (Adk), activities of the mutant Adk’s. It has also been used for the determination of glucose and ATP.
Biochem/physiol Actions
Hexokinase initiates the glycolysis pathway by catalyzing the phosphorylation of glucose to Glucose-6-phosphate in the presence of ATP. Glucose-6-phosphate dehydrogenase plays a role in the subsequent oxidation of Glucose-6-phosphate in the presence of triphosphopyridine nucleotide (TPN). Hexokinase and Glucose-6-phosphate Dehydrogenase both serve as a tool in biochemical and analytical studies for determining glucose, fructose, mannose, ATP, and creatin-kinase activity.
Packaging
Package sizes are based on hexokinase units.
Other Notes
Hexokinase Unit Definition: One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.
Mixture of hexokinase and glucose-6-phosphate dehydrogenase
1 of 1
This Item | |||
|---|---|---|---|
| description lyophilized powder | description Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian) | description Type VII, ammonium sulfate suspension, ≥200 units/mg protein | description lyophilized powder, ≥50 units/mg protein |
| form lyophilized powder | form lyophilized powder | form ammonium sulfate suspension | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| purified by crystallization | purified by crystallization | purified by - | purified by - |
| glucose-6-phosphate dehydrogenase activity ≥75 units/mg protein (biuret) | glucose-6-phosphate dehydrogenase activity - | glucose-6-phosphate dehydrogenase activity - | glucose-6-phosphate dehydrogenase activity - |
| hexokinase activity ≥65 units/mg protein | hexokinase activity - | hexokinase activity - | hexokinase activity - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the stability of a stock solution of Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast?
1 answer-
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What units are the package sizes of Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast, based upon?
1 answer-
This product is packaged on the basis of hexokinase units.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Does Sigma offer any other product which can be used in place of Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast?
1 answer-
Yes. We offer Product No. G3293 (Glucose Assay Reagent), which is a component of the GAHK20 (Glucose Assay Kit).
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How should Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast, be reconstituted?
1 answer-
Prior to determining the activity of this enzyme, Sigma reconstitutes it in 250 mM glycylglycine buffer, pH 7.4.
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Can Product H8629, Hexokinase and Glucose-6-phosphate Dehydrogenase from baker's yeast, be used for the determination of glucose?
1 answer-
A method for the determination of glucose using hexokinase and glucose 6-phosphate dehydrogenase is described in "Methods of Enzymatic Analysis" 3rd ed., Vol. VI, p. 163.
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