Key Documents

View All Documentation

Hepatocyte Growth Factor human

Name: Hepatocyte Growth Factor human
Brand: SIGMA
Price: 370 USD
Availability: InStock

≥95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

Synonym(s):

HGF, Hepatopoietin A, Scatter Factor

Select a Size

Change View

Size/SKU	Availability	Price
5 μg	Check Cart for Availability	$370.00
25 μg	Check Cart for Availability	$739.00

About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00217422

Biological source:

human

Recombinant:

expressed in NSO cells

Assay:

≥95% (SDS-PAGE)

Form:

lyophilized powder

Mol wt:

70-80 kDa by SDS-PAGE (non-reducing)

Impurities:

≤4 ng/mL (ED50)

$370.00

Check Cart for Availability

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

Hepatocyte Growth Factor human, HGF, recombinant, expressed in NSO cells, suitable for cell culture

biological source

human

Quality Segment

200

recombinant

expressed in NSO cells

assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

20-40 ng/mL

quality

endotoxin tested

mol wt

70-80 kDa by SDS-PAGE (non-reducing)

packaging

pkg of 5 and 25 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤4 ng/mL (ED50)

UniProt accession no.

P14210

storage temp.

−20°C

Gene Information

human ... HGF(3082)

Description

General description

Recombinant human Hepatocyte Growth Factor (HGF) is expressed in NSO cells and supplied as a lyophilized protein suitable for mammalian cell culture research. HGF is a cytokine that signals through the c‑Met receptor and is widely studied in in vitro cell models.

Application

Hepatocyte Growth Factor human has been used as a growth factor cocktail to suspend rat fibrogen/thrombin cells. It has also been used to study the tyrosine phosphorylation of MET (MET proto-oncogene, receptor tyrosine kinase) in myxofibrosarcoma cell line.

Biochem/physiol Actions

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Hepatocyte Growth Factor (HGF) is a multifunctional growth factor that exhibits mitogenic and motogenic activity in vitro and mediates cellular responses via c‑Met receptor signaling pathways.[1] It′s major function is to induce in vitro hepatocytes proliferation. HGF is also essential for cell migration and angiogenesis. Itis associated with embryonic development and tissue homeostasis. It is known to mediate placental trophoblast invasion, which is essential for normal fetal growth.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg of bovine serum albumin per 1μg of cytokine.

Analysis Note

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells.

Other Notes

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	K1757	H1404	H0652
Sigma-Aldrich H9661 Hepatocyte Growth Factor human	Sigma-Aldrich K1757 Keratinocyte Growth Factor human	Sigma-Aldrich H1404 Hepatocyte Growth Factor human	$Anti-Hepatocyte Growth Factor antibody produced in goat IgG fraction of antiserum, lyophilized powder$ Sigma-Aldrich H0652 Anti-Hepatocyte Growth Factor antibody produced in goat
description HGF, recombinant, expressed in NSO cells, suitable for cell culture	description KGF, recombinant, expressed in E. coli, powder, suitable for cell culture	description HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture	description IgG fraction of antiserum, lyophilized powder
biological source human	biological source human	biological source human	biological source goat
recombinant expressed in NSO cells	recombinant expressed in E. coli	recombinant expressed in Baculovirus infected High-5 cells	recombinant -
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) western blot: suitable, neutralization: suitable
assay ≥95% (SDS-PAGE)	assay ≥95% (SDS-PAGE and HPLC)	assay >95% (SDS-PAGE)	assay -
Quality Level 200	Quality Level 200	Quality Level -	Quality Level 100
form lyophilized powder	form powder	form lyophilized powder	form lyophilized powder

Still not finding the right product?

Explore all of our products under Hepatocyte Growth Factor human

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.