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I17001

Interferon-γ human

IFN-gamma, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin tested

Synonym(s):

IFN-γ

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Size/SKUAvailabilityPrice
100 μg

Available to ship TODAYfromMILWAUKEE

$465.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00131391
Form:
lyophilized powder
Assay:
≥98% (SDS-PAGE)
Recombinant:
expressed in HEK 293 cells
Mol wt:
16 kDa (glycosylated)

$465.00

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recombinant

expressed in HEK 293 cells

Quality Segment

assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

≤0.250 ng/mL In Viral Resistance Assay ED50

mol wt

16 kDa (glycosylated)

technique(s)

cell culture | mammalian: suitable

suitability

endotoxin tested

storage temp.

−20°C

General description

Interferon-γ (IFN-γ) is a pleotropic cytokine, encoded by the gene mapped to human chromosome 12q15. It is a non-covalent homodimer with two identical 17kDa polypeptide chains. It belongs to the type II class of interferons.
Recombinant human Interferon-γ (IFN-γ) is expressed in human 293 cells as a glycoprotein with a calculated molecular mass of 16 kDa. This protein is manufactured in human cells using an all-human production system, with full chemically defined ingredients and with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture. The bioactivity of IFN-γ expressed in human 293 cells is significantly higher compared to bacterially expressed IFN-γ.

Application

Interferon-γ human has been used in cytometric bead array to measure the levels of interferon-γ in the sample.
It has also been used for cytokine treatment of human islets to evaluate the activation of hypoxia inducible factor 1 α (HIF1α) targets, in vitro.

Biochem/physiol Actions

IFN-γ is a dimerized soluble cytokine that is the only member of the type II class of interferons.
Interferon-γ (IFN-γ) plays an essential role in function of virtually all immune cells and both innate and adaptive immune responses. IFN-γ exhibits various biological effects, such as antiviral activity, inhibition of cell or tumor growth and promotion of terminal differentiation of B cells into immunoglobulin-producing cells. This cytokine also activates macrophages, increases cytotoxicity of natural killer cells and promotes T cell cytotoxicity. In addition to antiviral activity, recombinant human IFN-γ is a potent modulator of immune responses and modifies cellular processes.

Physical form

Supplied as a lyophilized powder containing phosphate buffered saline.

Analysis Note

The biological activity of recombinant human IFN-γ was tested in culture in a viral resistance assay. The ED50 is defined as the effective concentration of IFN-γ that allows 50% cell growth in an antiviral cell based bioassay.

Other Notes

QDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRG

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This Item
SRP3058SRP3059I3265
Interferon-γ human IFN-gamma, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin tested

Sigma-Aldrich

I17001

Interferon-γ human

IFN-γ human Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), suitable for cell culture

Sigma-Aldrich

SRP3058

IFN-γ human

Interferon-λ human recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sigma-Aldrich

SRP3059

Interferon-λ human

Interferon-γ human recombinant, expressed in E. coli, ≥98% (SDS-PAGE), lyophilized powder, suitable for cell culture

Sigma-Aldrich

I3265

Interferon-γ human

description

IFN-gamma, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin tested

description

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), suitable for cell culture

description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), lyophilized powder, suitable for cell culture

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

suitability

endotoxin tested

suitability

-

suitability

-

suitability

-

mol wt

16 kDa (glycosylated)

mol wt

16.9 kDa

mol wt

19.8 kDa

mol wt

~17 kDa

form

lyophilized powder

form

lyophilized

form

lyophilized

form

lyophilized powder

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Storage Class

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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Certificates of Analysis (COA)

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Questions

1–2 of 2 Questions  

  1. How many Units are contained in 1 ug of this product?

    1 answer

    1. This product is supplied as a 100 uG pack size. The unit activity is reported on the lot specific Certificate of Analysis. See the link below to review a sample Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/I17001-100UG-PW0000210321.pdf

      For this specific lot, the reported activity is 76,000,000 units per milligram (1000 uG). The unit per microgram value for this lot is 76,000.

      Helpful?

  2. How the product I17001 can be reconstituted?

    1 answer

    1. Briefly centrifuge the vial before opening. It is recommended to reconstitute
      the protein in sterile, double-distilled water to a final concentration of 100 µg/ml.

      Please refer to this link for additional information about the product: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/579/551/i17001dat.pdf

      Helpful?

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