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I8896

IGEPAL® CA-630

Molecular Biology

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Synonym(s):

Octylphenoxy poly(ethyleneoxy)ethanol, branched

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50 mL

Available to ship TODAYfromMILWAUKEE

$70.90
100 mL

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$121.00

About This Item

Linear Formula:
(C2H4O)nC14H22O
CAS Number:
UNSPSC Code:
12352104
NACRES:
NA.31

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Quality Segment

grade

Molecular Biology

description

non-ionic

form

liquid

technique(s)

IR spectroscopy: suitable

CMC

0.08 mM (20-25°C)

viscosity

~250-350 cP(25 °C)

mp

6 °C

solubility

water: soluble at 20 °C

density

1.060 g/cm3

HLB

13

foreign activity

DNase and RNase, none detected

SMILES string

O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

General description

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40.

Application

IGEPAL® CA-630 has been used
  • as neutral detergent in homogenizing buffer during western blotting of mouse muscle homogenates
  • In X-gal staining for studying the lacZ expression in E8.5-E10.5 embryos
  • In Cell-Lysis (CL) buffer for preparing RT-qPCR-ready cell lysates

Analysis Note

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Other Notes

Nonionic, non-denaturing detergent

Legal Information

IGEPAL is a registered trademark of Syensqo

Disclaimer

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

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This Item
X305O01009001477502
description

Molecular Biology

description

70% in H2O

description

European Pharmacopoeia (EP) Reference Standard

description

United States Pharmacopeia (USP) Reference Standard

grade

Molecular Biology

grade

-

grade

pharmaceutical primary standard

grade

pharmaceutical primary standard

form

liquid

form

-

form

-

form

-

technique(s)

IR spectroscopy: suitable

technique(s)

blocking: suitable, cell culture | mammalian: suitable, protein purification: suitable, immunohistochemistry: suitable, hybridization: suitable

technique(s)

-

technique(s)

-

description

non-ionic

description

non-ionic

description

-

description

-

HLB

13

HLB

17.3

HLB

-

HLB

-

density

1.060 g/cm3

density

-

density

-

density

-


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signalword

Danger

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2



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Questions

1–10 of 17 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. In which solvent(s) is Atazanavir Sulfate soluble?

    1 answer
    1. Atazanavir Sulfate is soluble in DMSO: 104mg/mL and EtOH: 20mg/mL.

      Helpful?

  4. What is the storage temperature for this item?

    1 answer
    1. This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, the Safety Data Sheet (SDS).

      Helpful?

  5. Hi, I am enquiring about the storage temperature of product IGEPAL(R) CA-630 (I8896-100ml). The storage class is listed in the SDS but no temperature upper and lower limits. Would I be able to request this information. Thank you

    1 answer
    1. This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, and the Safety Data Sheet (SDS).

      Helpful?

  6. What is the molecular weight of Product No. I8896, IGEPAL® CA-630?

    1 answer
    1. The approximate molecular weight of this product is 603 Daltons. The average EO (ethylene oxide) units per molecule are 9.

      Helpful?

  7. What is the wt/vol % of this IGEPAL reagent? I want to use it for ATAC-seq and in the protocols people always mention the concentration of the stock is 10% wt/vol but I can't find this information on the specifications of this product. Thanks

    1 answer
    1. This product is provided as a neat liquid with a concentration of 100% which can be further diluted. The density of IGEPAL CA-630 is 1.06 g/cm3 at 25 °C. For a 10% wt./vol. solution 9.43 mL IGEPAL is needed per 100 mL of solution.

      The product is generally stable based on the chemical composition and a 10% (w/v) aqueous solution may be stable refrigerated for at least one month. However, it is important to minimize the exposure of the solution to oxygen (keep closed soon after use). The product does have a tendency to oxidize in air forming peroxides through a free radical process. In addition, some bacterial growth may occur which is evidenced by an odor or formation of a precipitate. Preservatives added to the solution may prevent bacterial growth. Please view the data sheet below: https://www.braskem.com.br/Portal/Principal/Arquivos/html/boletm_tecnico/PP%20Chemical%20Resistance.pdf

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. How does the storage temperature relate to shipping conditions?

    1 answer
    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      Helpful?

  10. How do we solubilize Product No. I8896, IGEPAL® CA-630?

    1 answer
    1. The product I8896 is miscible in all proportions with water.

      Helpful?

1–10 of 17 Questions  

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