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Interleukin-10 from rat

Name: Interleukin-10 from rat
Brand: SIGMA

>95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Synonym(s):

IL-10

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About This Item

UNSPSC Code:

12352202

EC Number:

201-064-4

NACRES:

NA.32

MDL number:

MFCD01635777

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Properties

biological source

rat

Quality Segment

100

recombinant

expressed in E. coli

assay

>95% (SDS-PAGE)

form

lyophilized powder

potency

1-35 ng/mL ED₅₀

mol wt

predicted mol wt ~19 kDa

packaging

pkg of 5 μg

impurities

endotoxin, tested

UniProt accession no.

P29456

storage temp.

−20°C

Gene Information

rat ... Il10(25325)

Description

Application

Interleukin-10 (IL-10) from rat has been used to study the anti-allodynic effect of IL-10 in rat model.

Biochem/physiol Actions

Interleukin-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1⁺ (CD5⁺⁾, and normal B cells. In human, the cellular sources of IL-10 consist of CD4⁺ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.

Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.

Interleukin-10 regulates lymphoid and myeloid cell functions; blocks the activation of cytokine synthesis and several accessory functions of macrophages; enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion.

Physical form

Lyophilized from a 0.2 μm filtered in 20 mM Tris, 0.1 M sodium chloride containing 50 μg of bovine serum albumin per 1 μg of cytokine.

Analysis Note

The biological activity is measured in a cell proliferation assay using the murine mast cell line, MC/9.

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This Item	I3019	I0531	I8648
Sigma-Aldrich I9154 Interleukin-10 from rat	Sigma-Aldrich I3019 Interleukin-10 from mouse	Sigma-Aldrich I0531 Interleukin-18 from rat	Sigma-Aldrich I8648 Interleukin-15 human
description >95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder	description >97% (SDS-PAGE), recombinant, expressed in E. coli, suitable for cell culture	description >97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder	description recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biological source rat	biological source mouse	biological source rat	biological source human
assay >95% (SDS-PAGE)	assay >97% (SDS-PAGE)	assay >97% (SDS-PAGE)	assay >97% (SDS-PAGE)
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
Quality Level 100	Quality Level 200	Quality Level 200	Quality Level 100
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

Storage Class

11 - Combustible Solids

wgk

WGK 3

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