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Lipopolysaccharides from Escherichia coli K-235

Name: Lipopolysaccharides from <I>Escherichia coli</I> K-235
Brand: SIGMA

purified by gel-filtration chromatography

Synonym(s):

LPS

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About This Item

CAS Number:

93572-42-0

UNSPSC Code:

12352201

EC Number:

297-473-0

NACRES:

NA.25

MDL number:

MFCD00164401

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Properties

biological source

Escherichia coli (K-235)

Quality Segment

200

form

lyophilized powder

purified by

gel-filtration chromatography

impurities

<1% Protein

color

white to yellow cast

solubility

water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow

shipped in

ambient

storage temp.

2-8°C

Description

General description

This product is extracted from E. coli serotype K235 and purified by gel filtration. The source strain is ATCC 13027.

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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1 of 1

This Item	L2143	L2762	L2637
Sigma-Aldrich L2018 Lipopolysaccharides from Escherichia coli K-235	Sigma-Aldrich L2143 Lipopolysaccharides from Escherichia coli K-235	Sigma-Aldrich L2762 Lipopolysaccharides from Escherichia coli O26:B6	Sigma-Aldrich L2637 Lipopolysaccharides from Escherichia coli O55:B5
biological source Escherichia coli (K-235)	biological source Escherichia coli (K-235)	biological source Escherichia coli (O26:B6)	biological source Escherichia coli (O55:B5)
solubility water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow	solubility water: soluble	solubility water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow	solubility water: soluble
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
impurities <1% Protein	impurities <3% Protein (Lowry)	impurities <5% Protein	impurities <3% Protein

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pictograms

GHS06

signalword

Danger

hcodes

H300

pcodes

P264 - P270 - P301 + P310 - P405 - P501

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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