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L2145

L-Leucine-7-amido-4-methylcoumarin hydrochloride

fluorogenic, ≥98% (TLC), powder

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Synonym(s):

H-L-Leu-AMC HCl

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5 mg
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$122.00
25 mg
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$399.00
100 mg
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$1,200.00

About This Item

Empirical Formula (Hill Notation):
C16H20N2O3 · HCl
CAS Number:
Molecular Weight:
324.80
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32
MDL number:

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Product Name

L-Leucine-7-amido-4-methylcoumarin hydrochloride,

assay

≥98% (TLC)

Quality Segment

form

powder

solubility

methanol: 50 mg/mL, clear, colorless to faintly yellow

fluorescence

λex 327 nm; λem 349 nm (pH 8.0), λex 380 nm; λem 440 nm (Reaction product)

storage temp.

−20°C

SMILES string

Cl.CC(C)C[C@H](N)C(=O)Nc1ccc2C(C)=CC(=O)Oc2c1

InChI

1S/C16H20N2O3.ClH/c1-9(2)6-13(17)16(20)18-11-4-5-12-10(3)7-15(19)21-14(12)8-11;/h4-5,7-9,13H,6,17H2,1-3H3,(H,18,20);1H/t13-;/m0./s1

InChI key

VCRXITKKWBOQRZ-ZOWNYOTGSA-N

General description

L-Leucine-7-amido-4-methylcoumarin hydrochloride (Leu-AMC) is a fluorogenic peptidyl substrate for leucine aminopeptidase, an extracellular enzyme found abundantly in natural aquatic systems. This enzyme is one of the various enzymes used by bacteria for protein hydrolysis.

Application

L-Leucine-7-amido-4-methylcoumarin hydrochloride has been used:
  • as a substrate in fluorescence-based soil assays
  • to determine leucine aminopeptidase activity of both Plasmodium falciparum M1 (PfA-M1) and PfA-M17 enzymes
L-Leucine-7-amido-4-methylcoumarin hydrochloride (Leu-AMC) as a substrate analog to determine leucine aminopeptidase activity

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This Item
A3401L2158B9385
description

-

description

protease substrate

description

≥98% (HPLC)

description

-

assay

≥98% (TLC)

assay

≥98% (TLC)

assay

≥98% (HPLC)

assay

≥98% (TLC)

solubility

methanol: 50 mg/mL, clear, colorless to faintly yellow

solubility

ethanol: 20 mg/mL, clear, colorless to light yellow

solubility

water: 25 mg/mL

solubility

water: 20 mg/mL, clear, colorless

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

fluorescence

λex 380 nm; λem 440 nm (Reaction product), λex 327 nm; λem 349 nm (pH 8.0)

fluorescence

-

fluorescence

-

fluorescence

-


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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