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L2380

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

lyophilized powder

Synonym(s):

Bandeirea simplicifolia agglutinin, BS-I

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Size/SKUAvailabilityPrice
1 mg
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$74.00
5 mg

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$246.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder

$74.00


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form

lyophilized powder

Quality Segment

potency

<20 μg per mL agglutination activity (using human blood group B erythrocytes)

composition

Protein, ≥90% biuret

impurities

salt, free

storage temp.

2-8°C

Biochem/physiol Actions

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Preparation Note

Purified by affinity chromatography using the method of Hayes, C. E., and Goldstein, I. J., J. Biol. Chem., 249, 1904 (1974).

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Other Notes

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

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This Item
L2140L9640L5505
description

lyophilized powder

description

Isolectin B4 (BSI-B4), biotin conjugate, lyophilized powder

description

lyophilized powder

description

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

impurities

salt, free

impurities

-

impurities

salt, essentially free

impurities

salt, essentially free

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

potency

<20 μg per mL agglutination activity (using human blood group B erythrocytes)

potency

<50 μg per mL agglutination activity (using human blood group B erythrocytes)

potency

<20 μg/mL agglutination activity

potency

<8 μg/mL agglutination activity

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

composition

Protein, ≥90% biuret

composition

Protein, ~95% E1%/280

composition

Protein, 100% biuret

composition

Protein, ≥80% biuret


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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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