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Lysozyme chloride form from chicken egg white

Name: Lysozyme chloride form from chicken egg white
Brand: SIGMA

Grade VI, ≥35,000 units/mg protein (E1%/282)

Synonym(s):

Peptidoglycan iV-acetylmuramoyl hydrolase, Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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About This Item

CAS Number:

9066-59-5

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-954-0

MDL number:

MFCD00131557

EC Number:

3.2.1.17 (BRENDA, IUBMB)

Specific activity:

≥35,000 units/mg protein (E1%/282)

Biological source:

chicken egg white

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Properties

biological source

chicken egg white

Quality Segment

200

type

Grade VI

form

lyophilized powder

specific activity

≥35,000 units/mg protein (E1%/282)

mol wt

~14.3 kDa

composition

Protein, ≥80%

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

application(s)

cell analysis

storage temp.

−20°C

Description

General description

Lysozyme, also known as muramidase, is a bacteriolytic enzyme found in phagocytic cells like leukocytes. The name "lysozyme" is derived from its ability to dissolve or hydrolyze the β-1-4-glycosidic bond between N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) in the walls of certain bacteria. This protein is present in animals, plants, bacteria, and viruses. It can also be found in granules of neutrophils, macrophages, as well as in serum, saliva, milk, honey, and hen egg white. There are different types of lysozymes, including the c-type (chicken type), the g-type (goose-type), and the i-type (invertebrates).

Application

Lysozyme chloride form from chicken egg white has been used:

as a component in the protein mixture to study the effects of yeast cells on membrane fouling caused by a protein mixture in dead-end filtration
in dip system to study its bacteriostatic or bactericidal effects on Salmonella-contaminated broiler parts
in viscometric assay for determining lysozyme activity

Biochem/physiol Actions

Lysozyme serves as an indicator of macrophage-mediated host response, correlates with white cell death, and exhibits a high turnover rate. Elevated levels of serum lysozyme have been observed in various chronic inflammatory conditions, inflammatory bowel diseases, hematological disorders, and renal disorders. The c-type lysozyme from hen egg white is commonly used as a model for studying protein structure and function. Muramidase primarily exhibits bactericidal activity against Gram-positive bacteria.

Physical form

Lyophilized powder containing sodium chloride and sodium acetate

Preparation Note

3× Crystallized

Other Notes

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

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This Item	L4631	62971	62970
Sigma-Aldrich L2879 Lysozyme chloride form from chicken egg white	Sigma-Aldrich L4631 Lysozyme from chicken egg white	Sigma-Aldrich 62971 Lysozyme from chicken egg white	Sigma-Aldrich 62970 Lysozyme from chicken egg white
biological source chicken egg white	biological source chicken egg white	biological source chicken egg white	biological source chicken egg white
specific activity ≥35,000 units/mg protein (E1%/282)	specific activity -	specific activity 70000-140000 U/mg	specific activity ~100000 U/mg
technique(s) cell based assay: suitable	technique(s) -	technique(s) cell based assay: suitable	technique(s) cell based assay: suitable
form lyophilized powder	form powder	form powder (crystalline)	form powder
application(s) cell analysis	application(s) -	application(s) cell analysis	application(s) cell analysis
suitability suitable for cell lysis	suitability -	suitability suitable for cell lysis	suitability suitable for cell lysis

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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