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L2895

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Isolectin B4 (BSI-B4), FITC conjugate, lyophilized powder

Synonym(s):

Bandeirea simplicifolia agglutinin, BS-I

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0.2 mg

Available to ship TODAYfromMILWAUKEE

$186.00
1 mg

Available to ship TODAYfromMILWAUKEE

$636.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder

$186.00

Available to ship TODAYDetails

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conjugate

FITC conjugate

Quality Segment

form

lyophilized powder

potency

<50 μg per mL agglutination activity (using human blood group B erythrocytes)

composition

Protein, ≥70% Lowry (, balance sodium citrate)

extent of labeling

≥1 mol FITC per mol lectin

storage temp.

2-8°C

General description

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.

Application

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used for microglia detection in murine astrocytes culture by staining the culture with lectin BS1-B4. It has also been used in the preparation of staining solution to incubate the tissue sections.

Biochem/physiol Actions

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling, because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.

Packaging

Package size based on protein content by Lowry.

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Other Notes

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

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This Item
L9381L2140L3019
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) Isolectin B4 (BSI-B4), FITC conjugate, lyophilized powder

Sigma-Aldrich

L2895

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) FITC conjugate, lyophilized powder

Sigma-Aldrich

L9381

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) Isolectin B4 (BSI-B4), biotin conjugate, lyophilized powder

Sigma-Aldrich

L2140

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) Isolectin B4 (BSI-B4), lyophilized powder

Sigma-Aldrich

L3019

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

description

Isolectin B4 (BSI-B4), FITC conjugate, lyophilized powder

description

FITC conjugate, lyophilized powder

description

Isolectin B4 (BSI-B4), biotin conjugate, lyophilized powder

description

Isolectin B4 (BSI-B4), lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

biotin conjugate

conjugate

-

potency

<50 μg per mL agglutination activity (using human blood group B erythrocytes)

potency

<100 μg per mL agglutination activity (using human blood group A erythrocytes)

potency

<50 μg per mL agglutination activity (using human blood group B erythrocytes)

potency

≥125 μg per mL agglutination activity (using human blood group A erythrocytes), ≤8 μg per mL agglutination activity (using human blood group B erythrocytes)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

extent of labeling

≥1 mol FITC per mol lectin

extent of labeling

1-3 mol FITC per mol protein

extent of labeling

3-6 mol biotin per mol protein

extent of labeling

-

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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